VALIDATION OF MOLECULAR MARKERS LINKED TO PHYTOPHTHORA ROOT ROT AND ROOT KNOT NEMATODE RESISTANCE LOCI IN SEGREGATING POPULATIONS OF CHILLI (Capsicum annuum L.)
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Date
2021
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Indira Gandhi Krishi Vishwavidyalaya, Raipur
Abstract
Chilli or hot pepper (Capsicum annuum L.) is one of the most important Solanaceous vegetable crops grown extensively in tropical and sub-tropical parts of the world. However, the commercial production of chilli is limited by various biotic stresses, among soil borne fungal diseases, Phytophthora root rot caused by Phytophthora capsici posing serious threats to chilli cultivation in regions with high humidity and rainfall. The obligate plant parasitic root knot nematode Meloidogyne species (M. incognita) causes characteristic galls and retards the growth of the plants. So identification of the molecular markers and their validation at the seedling stage mainly helps in chilli improvement programme. The present study was undertaken to validate the molecular markers linked to Phytophthora root rot and root-knot nematode resistance loci in different segregating (BC1, F1) populations of chilli. The experiments were conducted at the ICAR-Indian Institute of Horticultural Research, Bengaluru, Karnataka, during October 2020 to May 2021. In the present study 120 plants of BC1F1 [(IHR3291 x Arka Mohini) x IHR3291], 150 plants of BC1F1 IHR3288 x (IHR3288 x IHR3291) and 150 plants of F2 population derived from the cross IHR3291 x Arka Mohini were evaluated for Phytophthora root rot resistance under artificial epiphytotic condition against the virulent local IIHR isolate (gene bank accession number MK256294). 30 plants of BC1F1 [(IHR3291 x Arka Mohini) x IHR3291], 22 plants of BC1F1 IHR3288 x (IHR3288 x IHR3291) and 32 plants of F2 population derived from the cross IHR3291 x Arka Mohini were found phenotypically resistance against Phytophthora root rot. The phenotypically resistant plants were again validated /genotyped with the five molecular markers linked to the Phytophthora root rot resistance viz., HpmsE034, HpmsE062, CAMS405, SSR9 and CAMS839 and the plants were forwarded to next generation based on the homozygous loci for all the five markers. Among eighty-four phenotypically resistant plants of different population, thirty two plants were shown both phenotypic and genotypic resistance against Phytophthora root rot and the plants were even validated with the two molecular markers linked to the root knot nematode resistance viz., SCAR_N and SCAR_CD and their PCR amplification data was recorded. The plants which have shown combined resistance to both Phytophthora root rot and root-knot nematode were forwarded to next generation.
Description
VALIDATION OF MOLECULAR MARKERS LINKED TO PHYTOPHTHORA ROOT ROT AND ROOT KNOT NEMATODE RESISTANCE LOCI IN SEGREGATING POPULATIONS OF CHILLI (Capsicum annuum L.)