Phytochemical profiling and genetic diversity analysis of Mangifere indica Linn. varieties of Kerala
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Date
2020
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Department of Biotechnology, College of Agriculture, Vellayani
Abstract
The study entitled “Phytochemical profiling and genetic diversity analysis of Mangifera indica Linn. varieties in Kerala” was carried out at the Phytochemistry and Phytopharmacology Division of Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram during the academic year 2019 – 2020. The objective of the study was to map and authenticate selected local varieties of Mangifera indica in Kerala through phytochemical profiling of leaf volatile chemicals and the genetic diversity analysis using SCoT marker polymorphisms. Essential oils were obtained from the mature leaves of ten local mango varieties through hydrodistillation using a Clevenger-type apparatus and were analysed using GC-MS. A total of 55 volatile compounds ranging from 92.2 % to 98.0 % were identified from the ten varieties studied. Monoterpenes and sesquiterpenes were the major class of compounds among the identified volatiles. The prominent ones from this terpene classes include bicyclogermacrene, α-gurjunene, α-pinene, α-phellandrene, E-caryophyllene, terpinolene, δ-3-carene, limonene, α-humulene, germacrene D, β-selinene and germacrene B. While the statistical analysis of the varieties using their phytochemical composition clustered Vellari and Kilichundan as the nearest neighbours, Moovandan and Pulinji emerged as two distinct varieties from others in comparison. SCoT Primers proved to be a fine tool in studying the genetic diversity of M. indica varieties with an average polymorphism of 82% among the ten studied varieties. The varieties Kilichundan, Moovandan, Neelam and Kottoorkonam appeared as the ones with highest genetic similarity. Though the phytochemical and genetic diversities observed among the varieties are not directly linkable, the analyses helped to establish fine distinction between the varieties.
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PhD