Genetic analysis of Indian × East European F2:3 cross for yield traits and erucic acid in Brassica juncea (L.) Czern. & Coss
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Date
2021-07
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
A study was conducted on Brassica juncea (L.) Czern. & Coss. to identify Indian type low erucic acid genotypes from a segregating F2:3 cross between Indian genotype (Pusa Mehak) × East European genotype (Primus). Morphological data analyses indicated that there was a wide distribution of trait values obtained for F2 and F3 population. Correlation analyses among morphological traits revealed that the yield per plant in F3 population was significantly positively associated with plant height, number of secondary branches per plant and number of siliqua on main shoot and significantly negatively correlated to days to flowering. Cleaved Amplified Polymorphic Sequences (CAPS) based marker-assisted selection (MAS) was used for the selection of desirable genotypes. The PCR product of three CAPS markers namely CAPS591, CAPS1265 and CAPS237 were digested with restriction enzymes Hpy99 I, BGl II and Mnl I, respectively to group genotypes based on erucic acid content at the two paralogs of FAE gene, FAE1.1 and FAE1.2. The Indian type genotypes obtained through morphological selection based on early flowering, revealed the genetic constitution of the 175 genotypes at both loci. Based on the molecular profiling, six groups based on genotypic constitution at both loci were obtained, corresponding to erucic acid content. To validate the findings of MAS, the seeds of 175 F3 genotypes were subjected to biochemical analyses for erucic acid content. Associating the molecular data with biochemical analyses revealed seven genotypes [PMP3-152 (17.45%), PMP3-160 (18.49%), PMP3-218 (20.76%), PMP3-50 (22.11%), PMP3-101 (22.26%), PMP3-240 (22.40%) and PMP3-51 (22.59%)] with erucic acid towards the lesser side of the spectrum. The observed seven genotypes would form a valuable resource for developing low erucic acid Indian type genotypes in future breeding programmes.
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Preferred for your workSingh, Tushadri(2021). Genetic analysis of Indian × East European F2:3 cross for yield traits and erucic acid in Brassica juncea (L.) Czern. &Coss. MSc. Thesis submitted to Division of Plant Breeding and Genetics,Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, India