LINE × TESTER ANALYSIS FOR HORTICULTURAL TRAITS IN WHITE FRUITED BRINJAL (Solanum melongena L.)
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Date
2021-05
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UHF,NAUNI
Abstract
ABSTRACT
The present study entitled “Line × Tester analysis for horticultural traits in white fruited brinjal (Solanum
melongena L.)” was carried out during the Kharif, 2019 and 2020 at the Experimental Farm, Department of Vegetable
Science, College of Horticulture, Dr YSPUH&F, Nauni-173 230, Solan (HP), India to elucidate the information on the
extent of mean performance, heterosis, combining ability, nature and magnitude of gene action for various
horticultural traits. The experimental material consisted of 35 genotypes comprising of eight diverse lines, three
testers, 24 F1 hybrids resulted from employing Line × Tester mating design. The genotypes were evaluated in a field
trial conducted in Randomized Block Design with three replications during Kharif, 2020. The observations were
recorded on days to 50% flowering, days to first picking, number of fruits per plant, fruit length (cm), fruit breadth
(cm), fruit weight (g), number of leaves per plant, plant height (cm), number of primary branches per plant, stem girth
(cm), fruit yield per plant (g), projected fruit yield per hectare (q/ha), infestation of shoot and fruit borer (%),
incidence of fruit rot (%), TSS (ºB), ascorbic acid content (mg/100g), total phenol content (mg/100g) and antioxidant
activity (μmol TE/g FW). Further, 30 different SRAP marker combinations were used to determine the molecular
diversity among the parental genotypes used in the hybridization programme. The Line × Tester analysis revealed that
all the genotypes possessed wide spectrum of variability and showed significant differences for lines, testers and line
× tester interaction for majority of the traits studied. The white fruited brinjal varieties, Indira Safed Bangan (297.03
q/ha) followed by KKM-1 (252.82 q/ha), Kashi Himani (249.73 q/ha) and Shweta (242.24 q/ha) whereas, cross
combinations KKM-1 × Kashi Himani (397.40 q/ha), Nadia Local × Kashi Himani (392.41 q/ha), IC-090696 × Indira
Safed Bangan (389.23 q/ha) and IC-090696 × Shweta (354.52 q/ha) recorded the high fruit yield. These hybrid
combinations exhibited mid parent heterosis ranging from 58.15% (KKM-1 × Kashi Himani) to 79.27% (Nadia Local
× Kashi Himani) and heterobeltiosis ranging from 31.04% (IC-090696 × Indira Safed Bangan) to 57.19% (KKM-1 ×
Kashi Himani) for fruit yield. For yield and component traits, the parental lines viz., IC-090696, KKM-1, EC-169089
and Nadia Local had exerted good GCA effects whereas, Kashi Himani is the tester genotype with good GCA effect
for fruit yield. These genotypes with high GCA can be used in multiple crosses and their segregating population.
Among the cross combinations, the top four high yielding hybrid combinations with significant positive SCA effects
for fruit yield are Nadia Local × Kashi Himani (186.69), IC-112901 × Kashi Himani (132.78), KKM-1 × Kashi
Himani (112.40) and IC-090696 × Shweta (114.01). The genetic diversity studies using SRAP markers revealed that,
among the 30 SRAP primer combinations, 25 primer combinations were found to be polymorphic thus, appeared to be
useful to elucidate the molecular diversity in the parental genotypes. The number of band position generated by these
thirty markers for all the 11 parental genotypes were 101, which gave an average of 3.36 alleles per marker. The
dendrogram constructed from SRAP marker data divided eleven parental genotypes into three clusters. The molecular
diversity analysis carried out during the present investigation proved that, SRAP markers could be efficiently applied
to detect polymorphism even with a relatively low number of alleles.