“EFFECT OF GIBBERELIC ACID, POTASSIUM NITRATE AND SILICIC ACID ON BIOCHEMICAL CHANGES IN COWPEA (Vigna unguiculata L. Walp) SEEDLING IRRIGATED WITH SALINE WATER” 2862
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Date
2019-07
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JAU, JUNAGADH
Abstract
Green house experiment was conducted to investigate the effect of exogenous
application of gibberellic acid, potassium nitrate and silicic acid undersalt stress on
physiological, biochemical parameters and enzymatic activities of cowpea. The
experimental design was factorial completely randomized design with different
eight(T1 to T8) treatment combinations, twolevel of irrigation to induce salinity stress
i.e.tap water (S1) and 4 EC (S2). The plants were sprayed with GA3, KNO3 and silicic
acid after 10 and 30 DAS (Days after sowing) and samples with drawn after 20 and 40
DAS for the analysis. The observations were recorded for the parameters viz., relative
water content, leaf pH, membrane stability index, total phenol, true protein, free
amino acid, total soluble sugar, reducing sugar, chlorophyll content, carotenoids,
proline, glycine betaine as well as different enzyme viz., polyphenol oxidase,
peroxidase, nitrate reductase and phenylalanine ammonialyase..
The present investigation was under taken with an objective to find out the
effect of exogenous application of gibberellic acid, potassium nitrate and silicic acid
as a growth regulators on physiological, biochemical and enzymatic activates. Results
revealed that the foliar spray of treatment T8 [GA3@ 100 ppm + KNO3@ 500 ppm +
silicic acid@ 50 ppm] was more effective in all parameters.
In present study, it was observed that increasing the salt concentration in
treatments resulted to increase osmolytes or solutes viz.,total soluble sugar, reducing
sugar,glycine betain, total phenols, chlorophyll b, carotenoid and proline content incowpea under salt stress.With progressive days of germination, osmolytes or solute
content in the seedling decrease in all salt stress treatments.A difference in sensitivity
to salinity in cowpea was also reflected on physiological parametersas well as for the
enzymatic activity.Salt stress that decreased physiological parameters like; relative
water content andmembrane stability index but leaf pH is increase in salt stress.
The leaf sample that treated with gibberellic acid, potassium nitrate and silicic
acid at different concentration that increase physiological parameters like relative
water content,membrane stability index, leaf pH. GA3, KNO3, silicic acid possibly
regulate various plant metabolic process and modulate the production of varied
osmolytes like free amino acid, total soluble sugar, reducing sugar, chlorophyll
content (a and total), proline, enzyme activity like nitrate reducatse decreased under
salt stress, however, the application of GA3, KNO3 and silicic acid increase
peroxidise, nitrate reductase, phenylalanine ammonialyase, polyphenol oxidase
activity.
This investigation has proved GA3, KNO3 and silicic acid as a potential
biomolecules in reducing the adverse effect of salt stress in plant. GA3, KNO3 and
silicic acid has been showed to be beneficial for plant growth and development.