CULTURAL AND MORPHOLOGICAL CHARACTERIZATIONS OF Metarhizium anisopliae.
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Date
2019-07-23
Authors
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Publisher
Dr. Punjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.
Abstract
Metarhizium anisopliae is characterized as green muscardine fungus due to green colour of the sporulating colonies. M. anisopliae is an entomopathogenic fungus, used as important natural biocontrol agent against many insect pests throughout the world. It causes the death of insect due to the infective peg penetrate in the layer of insects integument and obliteration of tissues also production of toxins and proteolytic enzymes by the fungus.
The present study was aimed to isolate M. anisopliae from the infected insects of this region and evaluate the cultural and morphological characters of the fungus. Total ten isolates were collected from the infected insects and from market products. The isolates were identified by testing growth characters on media and morphological characters. The white, prominently raised mycelial growth became greenish on sporulation on the medium. The conidiophores were errect, branched covered by conidia, the conidia were apical long single celled ovoid to cylindrical with rounded end. These isolates were tested on Spodoptera litura larvae for pathogenicity of M. anisopliae fungus. All the isolates showed variable mortality percentage and maximum mortality was noticed by Ma2 (90%) in S. litura larvae. Further confirmation of efficacy of spore suspension was assessed by leaf dip method and it was observed that the mortality was rapidly increased upto 91.66 per cent in spore concentration of 1 x 109 spore/ml on 10th days of incubation. The rate of mortality was reduced with reduction in the spore concentration.
Therefore Ma2 isolates was taken for further studies. Cultural and morphological studies of M. anisopliae was taken on four different media viz., Potato Dextrose Agar (PDA), Sabouraud Dextrose Agar (SDA), Sabouraud’s Dextrose Agar + Yeast Extract (SDAY) and Sabouraud Maltose Agar (SMA) medium. SDAY medium support maximum growth and sporulation of M. anisopliae at 250C temperature, 95 per cent relative humidity and 5.5 pH levels. Compatibility studies were assessed with four insecticides viz., Imidacloprid, Spinosad, Chloropyriphos and Indoxacarb. Out of these M. anisopliae slightly compatible with spinosad and imadacloprid at normal concentration dose of insecticide.
Description
Metarhizium anisopliae it is a entomopathogenic fungi. Isolates of Metarhizium anisopliae were collected and studied its cultural and morphological characterizations on four different media viz., PDA, SDA, SDAY and SMA medium. SDAY medium was selected as a basal medium for further studies on different environmental factors like temperature, humidity and pH. Also pathogenicity and efficacy of spore suspension was assessed on Spodoptera litura larvae by leaf dip method then Compatibility studies of Metarhizium anisopliae were assessed with different insecticides by poison food technique.
Keywords
Biological control agent, Insect pest control, Ecological control.
Citation
SONUNE, BHUSHAN DEVIDAS. (2019). Cultural and morphological characterizations of Metarhizium anisopliae. Department of plant pathology, Nagpur. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2019. Print. xii, 51p. (unpublished).