Studies on Alternative Sources in In-vitro Culture of Potato (Solanum tuberosum L.)

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Date
2021-03
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
The present investigation entitled “Studies on Alternative Sources in In-vitro Culture of Potato (Solanum tuberosum L.)” was conducted during the year 2019-20 with the objectives (i) To find out alternative gelling agents for low cost medium (ii) To find out alternative carbon sources for low cost medium and (iii) To assess feasibility of natural indirect light as alternative source for in vitro culture. Two explants from variety Kufri Girdhari viz., sprouts and nodal segments were used for the experiment after sterilization with NaOCl (2%). For shoot induction, ten different concentrations of BAP (0.0, 0.25, 0.5, 0.75, 1.0 and 2.0 mg/l) and kinetin (0.0, 0.25, 0.5 0.75 and 1.0 mg/l) were used. Amongst all the treatments, significantly superior response with good quality compact, dark green primordia was observed in treatment T4- MS + 0.75 mg/l BAP manifested in terms of early days to shoot induction in sprout explants (9.58) as well as nodal segment explants (7.43) and higher percentage response in case of sprout explants (87.06 %) as well as nodal segment explants (85.76 %). For shoot multiplication, thirteen different concentrations of BAP (0.0, 0.25, 0.5, 0.75, 1.0, 1.5 & 2.0 mg/l) alone and in combination with kinetin (0.25 mg/l) were used. Amongst all the treatments significantly superior response in terms of number of shoots per explant (15.50), average length of shoots (8.13 cm) and internodal length (1.12 cm) was recorded from treatment T10 - MS + 0.75 mg/l BAP + 0.25 mg/l kinetin. For induction of rooting, healthy in vitro multiplied shoots were cultured on MS medium supplemented with different concentrations of IBA (0.0, 0.25, 0.5, 1.0, 1.5 and 2.0 mg/l). Amongst all the treatments, T4 - MS + 1.0 mg/l IBA recorded superior responses in terms of percentage rooting (97.50 %), number of roots per shoot (8.50), average root length (6.97 cm), number of leaves per plantlet (15.79) and plant height (14.83 cm). The in vitro raised single shoots obtained from shoot multiplication stage were used during experiments on alternative gelling agents, carbon sources and light sources. For studies on alternative gelling agents five different combinations of Agar and Isabgol viz. 100 % Isabgol, 75% Isabgol + 25 % Agar, 50 % Isabgol + 50 % Agar, 25% Isabgol + 75 % Agar and 100 % Agar were used on optimised medium for shoot multiplication. The results revealed that the maximum number of shoots per explant was found in treatment T5- 100 % Agar (15.50) followed by T2 - 75% Isabgol + 25 % Agar (13.05). The highest average shoot length (8.20 cm) was found in treatment T2 - 75% Isabgol + 25 % Agar. In the study on alternative carbon source, viz. common table sugar, the results were at par or even better as compared to standard carbon source viz. sucrose. The results revealed maximum number of shoots (15.50), longer shoot length (8.13 cm) and longer internodal distance (1.12 cm) were observed in sucrose. The number of nodes per plantlet was maximum (9.02) in common table sugar. The percentage rooting response (98.17) and average root length (7.10 cm) was higher in common table sugar. Amongst the alternative light sources, natural indirect light was better alternative in terms of number of shoots (15.98), average shoot length (9.85 cm) and internodal distance (1.42 cm) as compared to fluorescent light. The natural indirect light also gave higher percentage rooting response (97.57) with higher response in terms of number of roots per shoot (9.20), average root length (7.03 cm) and plant height (15.36 cm).
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Preferred for your work. Walia, A. 2021. Studies on Alternative Sources in In-vitro Culture of Potato (Solanum tuberosum L.). M.Sc. thesis, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, India.
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