Evaluation of genotypes and micropropagation in turmeric (Curcuma longa L.)
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Date
2021-03
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Sher-e-Kashmir University of Agricultural Sciences and Technology Jammu, J&K
Abstract
The present investigation entitled “Evaluation of Genotypes and Micropropagation in Turmeric (Curcuma longa L.)” was conducted during the year 2019-20 with the objectives i) To evaluate the field performance of genotypes for yield and quality traits; and ii) To study micropropagation protocol. For field evaluation ten genotypes viz., SJT-09, Prabha, Sugandham, Suroma, Tanda local, Rajendra Sonia, PH-1, Allepey Supreme, Suguna and Local Chowkichora were used. The experiment was laid out in Randomized Block Design with three replications. The analysis of variance revealed significant differences among genotypes in almost all the characters. With respect to growth parameters maximum plant height was recorded in genotype Suroma (101.06cm), whereas maximum number of leaves per plant was recorded in Tanda Local (7.83). The maximum Leaf Area Index (LAI) was recorded for Suroma (7.43). The maximum number of fingers per rhizome (15.38) and length of fingers per rhizome (4.22 cm) was recorded in SJT-09 whereas, girth of fingers per rhizome (cm) was statistically non significant. In case of yield parameters the maximum rhizome yield per plot (kg) and rhizome yield per hectare (tonnes) was recorded in SJT-09 (18.38 kg and 20.39 t/ha respectively) whereas minimum crop duration was recorded in Prabha (201 days). With respect to quality parameters highest curcumin content (%) was recorded in Rajendra Sonia (6.43%) and maximum dry recovery (%) was in SJT-09 (20.60%).
For standardization of micropropagation protocol various concentrations and combinations of BAP and NAA were used. Fresh rhizome buds were used as explants and were sterilized with sodium hypochlorite (2%) and mercuric chloride (0.2%) followed by 5-7 times with sterile distilled water. For shoot bud initiation explants were cultured on MS medium supplemented with different concentration of BAP (1.0, 1.5, 2.0, 2.5 and 3.0 mg/l) and in combination with NAA (0.5mg/l). Among all the treatment combinations, significantly superior response was observed in T4- MS medium supplemented with 2.0 mg/l BAP for high percentage response (82.63%) and minimum days to shoot bud initiation (14.66). For shoot multiplication and rooting MS medium was supplemented with BAP (1.0, 1.5, 2.0 and 2.5 mg/l) in combination with NAA (0.5, 1.0 and 1.5mg/l). However, maximum number of shoots per explants (7.67) and average shoot length (10.46 cm) was recorded in T4 treatment- MS medium supplemented with 2.5 mg/l BAP and 0.5mg/l NAA whereas maximum rooting response (83.33%), number of roots per shoot (6.03) and average root length (7.46 cm) was recorded in T9 treatment i.e. MS medium supplemented with 1.0mg/l BAP and 1.5mg/l NAA. For hardening the plantlets were transferred to plastic pots containing Sand: Soil: FYM in 1:1:1 ratio for 3 weeks and kept under greenhouse conditions.
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Preferred for your work. Shailla Hussain. 2021. Seasonal Abundance and Management of Major Insect Pests of Linseed (Linum Usitattisimum L.). M.Sc. thesis, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, India.