Age Related Histomorphological, Histochemical and Ultrastructural Status of Lymphoid Organs in Japanese Quail (Conturnix coturnix japonica)
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Date
2020-11-25
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MAFSU, Nagpur
Abstract
The present study was carried out on 48 samples, each of spleen, bursa of
fabricius and thymus of Japanese quail of one to four week of age.
The histomorphological study was carried out from the tissue stained with
Haematoxylin and eosin, Massons’trichrome, Verhoeff’s elastic tissue stain, and
Gomori’s reticulin method. The histochemical observations were recorded for the
presence of glycogen and acid mucopolysachharides.
The tissues of spleen, bursa of fabricius and thymus were subjected for
transmission electron microscopy to confirm the cellular population and
ultrastructural study of cellular population of spleen, bursa of fabricius and
thymus at different age.
The age wise changes in the cellular population and cellular morphology
of spleen, bursa of fabricius and thymus were recorded. The increase in
macrophages, plasma cells and Hassall’s corpuscles was recorded.
The increase in the thickness of splenic capsule, diameter and number of
splenic nodule, number of ellipsoids, thickness of thymic capsule, diameter of
thymic lobule, thickness of thymic cortex, diameter of thymic medulla, number of
Hassall’s corpuscles, number and height of bursal mucosal fold, height of bursal
mucosal surface epithelium and diameter of bursal lymphoid follicle was noticed
with the advancement of age.
The histochemical reaction for presence of glycogen and acid
mucopolysaccharides varied from mild to intense in different components of
spleen, bursa of fabricius and thymus at different age.