Age Related Histomorphological, Histochemical and Ultrastructural Status of Lymphoid Organs in Japanese Quail (Conturnix coturnix japonica)

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Date
2020-11-25
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MAFSU, Nagpur
Abstract
The present study was carried out on 48 samples, each of spleen, bursa of fabricius and thymus of Japanese quail of one to four week of age. The histomorphological study was carried out from the tissue stained with Haematoxylin and eosin, Massons’trichrome, Verhoeff’s elastic tissue stain, and Gomori’s reticulin method. The histochemical observations were recorded for the presence of glycogen and acid mucopolysachharides. The tissues of spleen, bursa of fabricius and thymus were subjected for transmission electron microscopy to confirm the cellular population and ultrastructural study of cellular population of spleen, bursa of fabricius and thymus at different age. The age wise changes in the cellular population and cellular morphology of spleen, bursa of fabricius and thymus were recorded. The increase in macrophages, plasma cells and Hassall’s corpuscles was recorded. The increase in the thickness of splenic capsule, diameter and number of splenic nodule, number of ellipsoids, thickness of thymic capsule, diameter of thymic lobule, thickness of thymic cortex, diameter of thymic medulla, number of Hassall’s corpuscles, number and height of bursal mucosal fold, height of bursal mucosal surface epithelium and diameter of bursal lymphoid follicle was noticed with the advancement of age. The histochemical reaction for presence of glycogen and acid mucopolysaccharides varied from mild to intense in different components of spleen, bursa of fabricius and thymus at different age.
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