IMPACT OF DIFFERENT HARDENING SUBSTRATE ON GROWTH AND SURVIVAL RATE OF IN VITRO RAISED PLANTLETS OF ORCHIDS (Dendrobium nobile)
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Date
2020
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Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.)
Abstract
The present research “Impact of Different Hardening Substrate on Growth and Survival Rate of In Vitro Raised Plantlets of Orchids (Dendrobium nobile).” was undertaken with a view to analyse the effect of different hardening media at Poly house of Commercial Tissue Laboratory, College of Agriculture, Indira Gandhi Krishi Viswavidyalaya, Raipur, Chhattisgarh during 2019-20. The experiment layout has been kept in Completely Randomized Design (CRD) in combination of 16 treatments replicated thrice.
The results reveals that the highest value for parameters including height of plant, number of leaves plant-1, length of leaf, width of leaf, leaf area, leaf area index, survival % has been recorded in T8 (Chopped coconut husk + Brick + Charcoal + Cocopeat). The highest shoot girth has been observed in T2 (Bark + Brick + Charcoal + Cocopeat) and maximum number of root plant-1, length of root, root volume has been observed in T9 (Chopped coconut husk + Brick + Charcoal + Rice Husk).
Better outcome were obtained by the treatment T8 as it contained coconut husk, brick pieces, charcoal and cocopeat. Where, coconut husk is ideally suited for enhancing the water holding ability of the substrate and nutrient content at initial phase, brick pieces help to provide good aeration and mechanical support to plants, charcoal has also been effective in aerating and retaining fertilizer when pouring fertilizer solution and it continuously transfer nutrients to developing plants on subsequent watering, cocopeat carries eight times of water of its weight it improves water holding capacity of media and also releases nutrients over long periods. While treatment T10 gives minimum outcomes in almost all parameters might be due to the reason that medium containing chopped paddy straw, brick and charcoal could not supply enough support and nutrients required for the growth and also get compact after decomposition of paddy straw. Therefore the substrate combination T8 (Chopped coconut husk + Brick + Charcoal + Cocopeat) may be recommended for hardening substrate of orchids.
Description
IMPACT OF DIFFERENT HARDENING SUBSTRATE ON GROWTH AND SURVIVAL RATE OF IN VITRO RAISED PLANTLETS OF ORCHIDS (Dendrobium nobile)