STUDIES ON STANDARDIZATION OF PRODUCTION TECHNOLOGY OF LION’S MANE MUSHROOM (Hericium erinaceus) IN CHHATTISGARH
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Date
2020
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Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.)
Abstract
The present study entitled “Studies on standardization of production technology of Lion’s Mane Mushroom (Hericium erinaceus) in Chhattisgarh” was carried out in the Mushroom Research Laboratory, Department of Plant Pathology, College of Agriculture, Raipur, (Chhattisgarh).
In the present investigation, 8 media were evaluated among them potato dextrose agar and potato dextrose broth were most suitable for growth and biomass of both studied strain (HE-05 and HE-08). Maximum mycelia growth and biomass were observed at 24°C but minimum mycelial growth and biomass was recorded at 16°C and 32°C respectively. pH 6.0 gave more mycelia growth and biomass of both studied strains and no myceial growth was observed at pH 4.0. Fructose substituted medium gave more growth but higher biomass was noticed in medium containing maltose and glucose as carbon sources in HE-05 and HE-08 strains respectively. Among the nitrogen sources, ammonium per sulphate was found good as nitrogen source to enhance mycelial growth but highest biomass was occurred in glycine incorporated medium. The plates were exposed at 492-577nm produced more mycelia growth and biomass of both strains of H. erinaceus. Among the evaluated different substrate, saw dust gave better yield a other substrate. Earliest spawn development of H. erinaceus was observed on finger millet grains but wheat grains raised spawn produced higher yield as compare to other grains based spawn. The growth of H. erinaceus was inhibited 100% by formaldehyde, carbendazim, formaldehyde + carbendazim as compare to control while lime inhibit 38.44% and 22.44% growth of HE-05 and HE-08 respectively. Steam sterilized wheat straw gave maximum growth and yield of H. erinaceus. Nine strains were evaluated among them HE-02 strain was found most superior for growth while HE-08 produced more biomass on potato dextrose broth. On wheat extract medium HE-08 strain gave more growth and biomass. Maximum septa, clamp connection, width of wall (µm) and minimum distance between septa (µm), clamp connection (µm) per microscopic field were noticed in HE-02 and HE-08 strains as compare to other strains. Nine strains of H. erinaceus were screened, among them HE-08 was found superior and showed quicker spawn development, spawn run on substrate and produced highest yield on both studied substrate (wheat straw and saw dust). Different strains of H. erinaceus had moisture (80.30 – 85.93%), ash (7.26- 10.45%), protein (22.25-28.68%), total sugar (53.57- 61.89%), fat (2.00-2.91%) and fiber (5.54-6.85%. Poly propylene bag method was found most superior to obtain maximum (38.26%) biological efficiency of H. erinaceus.
Description
STUDIES ON STANDARDIZATION OF PRODUCTION TECHNOLOGY OF LION’S MANE MUSHROOM (Hericium erinaceus) IN CHHATTISGARH