DIAGNOSIS AND IDENTIFICATION OF VIRUS CAUSING MOSAIC DISEASE IN CAPSICUM (Capsicum annum var. grossum sendt)
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Date
2017-03
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University of Horticultural Sciences, Bagalkot (COLLEGE OF HORTICULTURE, UHS CAMPUS,GKVK POST, BANGALURU)
Abstract
An Investigation on “Diagnosis and identification of virus causing mosaic disease in capsicum (Capsicum annum var. grossumsendt)” was conducted at Department of Plant Pathology, College of Horticulture, UHS Campus, GKVK, Bengaluru during 2015-16. In the present investigation the survey report determined the incidence of mosaic disease in major capsicum growing districts like Chikkaballapura, Kolar, Bengaluru rural and Ramanagar. The maximum incidence of 54.85 per cent was found in Hosahalli village, Ramanagar district and minimum incidence of 7.99 per cent was found in Tammanayakanahalli village, Chikkaballapura district. DAC-ELISA results revealed that the forty samples collected from different districts showing vein banding, leaf distortion and mosaic mottling symptoms with composition of both CMV and ChiVMV. However, only ten samples showed reaction with CMV antiserum. Screening of seventeen entries of capsicum including varieties, hybrids and germplasm against ChiVMV disease resistance by sap inoculation revealed that, hybrids viz., Diana, Starlet, Aristotle and germplasm lines DCA 310 were found to be moderately resistant to ChiVMV and the hybrids viz., Sympathy, Inspiration, Pasarella, SV1865PB and four germpalasm lines DCA 311, DCA 355, DCA 322 and DCA 346 were found to be moderately susceptible. The host range study revealed that the plant species namely Nicotiana tabacum cv. Samsun, N. glutinosa, N. occidentalis, Datura metel, Physalis floridana, Solanum nigrum, Capsicum annum were known to be infected with the ChiVMV. In the study on vector transmission, five aphids per plant showed highest per cent transmission (100 %). The effect of different dates of inoculation on different plant growth parameters, the highest per cent disease transmission was observed in 15 days after sowing, inoculation immediately after planting, 15, 30, 45 days after planting and lowest in 90 days after planting. In phylogenetic analyses of the ChiVMV isolates, the nucleotide identity of the ChiVMV between Kolar and Chikkaballapura had 89 per cent sequence similarity. The highest nucleotide sequence similarity of Kolar was 93.81 per cent with ChiVMV of Raichur isolate (EF213688.1) and the lowest was 78.31 per cent with the ChiVMV of Pakistan isolate (KJ472764.1). However, the Chikkaballapura isolate had highest homology of 93.97 per cent with ChiVMV of Rajastan isolate (KJ000073.1) and lowest of 81.96 per cent with ChiVMV of Pakistan isolate (KJ472764.1).