FIELD EVALUATION FOR AN EARLY GENERATION GENETIC VARIABILITY AND SCREENING OF A DNA MARKER LINKED TO BACTERIAL WILT RESISTANCE IN BRINJAL (Solanum melongena L.)
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Date
2017-01
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University of Horticultural Sciences, Bagalkot (COLLEGE OF HORTICULTURE, GKVK POST, BANGALURU)
Abstract
Field and laboratory experiments were carried out during 2014-15 at Department of Vegetable Science, College of Horticulture, Bengaluru to study the early generation genetic variability and to screen the DNA marker linked to bacterial wilt resistance in brinjal population. The F2 generation derived from the crosses Green long × IIHR-3 and Raidurga Green Round × WCGR were used for evaluation of genetic variability, where Green Long and Raidurga Green Round were bacterial wilt susceptible parents with good horticultural traits and IIHR-3 and WCGR were used as resistant source to bacterial wilt. The F2 plants showed significant difference for all the characters studied. The phenotypic correlation coefficients between yield and plant height, number of branches per plant and number of fruits per plant were of higher magnitude in positive direction; while, highly significant correlation between yield has been observed for fruit length and breadth in Raidurga Green Round × WCGR. Similarly, a highly negative correlation between average yield per plant and days to first flowering has been recorded in both the crosses. Hence, selection programme through these component traits may help in improvement of yield. Later, 40 high yielding selfed F2 lines were selected and advanced to F3 generation for screening bacterial wilt resistance. In F3 population, eight lines namely 1, 18, 46, 50, 97, 124, 164 and 170 from Green Long × IIHR-3 and seven lines viz., 30, 67, 84, 86, 108, 233 and 249 from Raidurga Green Round × WCGR were found to be highly resistant to bacterial wilt which can be used in further generation for bacterial wilt resistance breeding. DNA marker of 762 bp reported from China found not linked to bacterial wilt resistance in Indian background, indicating genotypic specificity and possibility of diverse genetic make-up.