Analysis of Genetic Variation in Artocarpus hirsutus Lam. (wild jack) Collections from Western Ghats

Loading...
Thumbnail Image
Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Division of Plant Genetic Resources ICAR-Indian Agricultural Research Institute New Delhi
Abstract
A total of sixty-eight genotypes of wild jack (Family: Moraceae) were collected from Karnataka and Kerala states preferably from the Kaan Community Forest. Genotypes were collected from in-situ and ex-situ sites that were used to investigate morphological, biochemical and genetic variation among sub-population and individual genotypes. Additionally, the nutritional importance of seed and fruit were studied. Morphological data were recorded among sixty-eight genotypes of seed and kernel by using Morphometry software. The mean values were following in seed area 104.6mm2 , seed weight 0.43gm were recorded while the maximum sample variance found in seed area. Eight colour groups were found in kernel and the highest seventeen samples were grouped in pink (dark) colour. The mean value of fruit weight was 112.2gm and among total weight, 49% is edible portion including pulp and seed. In per 100gm of fruit pulp, mean value of total sugar 12gm, total antioxidant 1.9mg gallic acid equivalent (GAE), total phenol 0.44mg GAE, beta carotene 441μg, ascorbic acid 8.13mg, Total Starch 16.7gm were reported. Similarly, in seed flour moisture 47.5%, total phenol 2.5mg GAE, total antioxidant 2.74mg GAE, total sugar 7.3gm, total nitrogen 17.6%, total starch 17.3gm were found. A total of fifty-six genotypes from eight sub-population were selected for molecular analysis. Among seventy-seven SSR primer pairs forty-three were amplified and thirty were polymorphic markers. Average pairwise Jaccard’s similarity 0.59, overall expected heterozygosity was 0.33 and mean heterozygosity was 0.29. Overall Fst value for all locus is 0.11 which showed modest genetic differentiation between eight different populations of wild jack while gene flow (Nm) is estimated 2.07 respectively. The mean value of the observed number of alleles was 1.86 (n*), the mean value of the effective number of alleles (ne) was 1.68; the Mean value of the Shannon Information Index (I) is 0.49. Total of fifty-six genotypes were representative of eight different natural populations. Pop-1 and Pop-3 were clustered together in both dendrograms while Pop-2, Pop4, Pop-5, Pop-7 and Pop-8 clustered together while Pop-6 is distantly related among both dendrograms. Conclusively, wild jack is a keystone and endemic species and its importance for community, environment and ecosystem is imperative so its
Description
T-10402
Keywords
Citation
Collections