Rhizosphere mediated nutrient management in rice using phosphate solubilising rhizobacteria isolates in inceptisols
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Date
2020-12
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Sher-e-Kashmir University of Agricultural Sciences and Technology Jammu, J&K
Abstract
The present investigation entitled “Rhizosphere mediated nutrient management in rice using phosphate solubilising rhizobacteria in inceptisols” was carried out at Division of Soil Science and Agriculture Chemistry of Sher-e-Kashmir University of Agricultural Sciences and Technology. during 2019-2020. An attempt has been made for the identification of PSRB isolates isolated from the rice rhizosphere and to study the effect of PSRB isolates on nutrient availability, biological activity in rhizosphere soil and yield attributes of rice crop. 16S rRNA gene sequencing identified PSRB 1 as Pseudomonas aeruginosa and PSRB 2 as Bacillus subtilis. In-vitro growth conditions of PSRB isolates in Pikovskaya medium revealed Pseudomonas aeruginosa strain showed its maximum growth at (6.5 pH, 330C,1.5% salt conc.) and Bacillus subtilis strain showed its maximum growth at (7.5 pH, 370C, 2.0 % salt conc.) Qualitative estimation of phosphate solubilisation activity of PSRB isolates in Pikovskaya agar plates revealed high phosphate solubilization index of (2.82) with Pseudomonas aeruginosa. Quantitative estimation of phosphate solubilisation of PSRB isolates in Pikovskaya Broth was recorded highest in (Pseudomonas aeruginosa and Bacillus subtilis consortium) with 7.86 µg/ml of P release at 5th day interval. Pot trial was laid out in a Factorial CRD with 3 replications and six treatment combinations. Seven days old seedlings of rice variety Basmati 370 were applied with alone or in combination of TCP and PSRB isolates broth culture during the first week of July month. Application of TCP and consortium of PSRB isolates showed significant increase on available nutrients in rhizosphere soil of rice crop. Highest available N with (17.13%) at tillering stage, (19.1%) at panicle initiation stage, available P with (232%) at tillering stage, (265%) at panicle initiation stage and available K with (19.6%) at tillering stage, (29.2%) at panicle initiation stage were recorded with treatment B3 containing (Pseudomonas aeruginosa and Bacillus subtilis consortium). TCP and PSRB isolates consortium amended soil showed significant increase in SMBC and dehydrogenase activity. Highest SMBC with (16.4%) at tillering stage and (16.5%) at panicle initiation stage and highest dehydrogenase activity with (34.7%) at tillering stage, (43.8%) at panicle initiation stage were recorded in treatment B3 (Pseudomonas aeruginosa and Bacillus subtilis consortium). Maximum yield with (63.2%) and N uptake with (6.4%), P uptake with (15.8%) and K uptake with (8.9%) was recorded in treatment B3 (Pseudomonas aeruginosa and Bacillus subtilis consortium) in rice crop.
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Preferred for your work: Anshu. 2020. Rhizosphere mediated nutrient management in rice using phosphate solubilising bacteria in inceptisols.