CERTAIN EPIDEMIOLOGICAL ASPECTS OF ANTIBIOTIC RESIDUES IN CATTLE MILK AT SELECTED DISTRICTS OF KARNATAKA
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Date
2017-06
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
A total of 120 milch cows reared in organized and unorganized sectors were
screened for antibiotic residues in milk at Bangalore Rural, Kolar and Chickballapur
districts by non-probability sampling. Epidemiological data with respect to various
parameters were collected and recorded. Forty milk samples each from three districts
were subjected to different screening tests namely MDRT, microbiological method and
Beta-lactam ELISA. Out of the 120 samples screened, MDRT revealed 32 positive
samples (26.7%) and among these 27 (22.5%) had antibiotic equal to or more than MRL
and five (4.2%) had antibiotic less than MRL. By microbiological method 29 samples
(24.1%) showed positive results in the disc assay based on measuring the zone of
inhibition produced by Bacillus stearothermophilus. By ELISA 26 samples (21.7%) were
found to be positive for Beta-lactams with more than 4 ng/ml. Kolar district had the
maximum number of positive cases in all the above tests. A total of 35 (29.2%) samples
were tested positive for antibiotic residues either by one test or a combination of tests.
Among the positive milk samples nine were from Chickballapur district, 10 from
Bangalore Rural district and 16 from Kolar district. Among the total animals screened,
the number of cattle positive for all the three tests was 25 (20.8%), number of cattle
positive for any two test combinations was 26 (21.7%) and the number of cattle positive
for one test was found to be 32 by MDRT (26.7%), 29 by Microbiological method
(24.1%) and 26 by ELISA (21.7%). Statistical analysis of different tests in each district
was carried out by Chi square test and it was observed that no significant difference (P ≥
0.05) was present between the three tests in all the districts.