Studies on shared biochemical and molecular responses to biotic (fusarium wilt) and abiotic (drought) stresses in Chickpea(Cicer arientium L)
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Date
2015
Authors
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Mahatma Phule Krishi Vidyapeeth, Rahuri
Abstract
The present investigation entitled “Studies on
shared biochemical and molecular responses to biotic (Fusarium wilt) and
abiotic (drought) stresses in chickpea (Cicer arietinum L.)” was
undertaken to evaluate the activity profile of defense related enzymes and
antioxidative enzymes during chickpea-Fusarium interaction and to
correlate these changes with field infestation of chickpea in wilt sick soil.
Total thirty one chickpea genotypes including four wilt resistant and
three wilt susceptible checks were evaluated for total phenols and activity
profile of defense related enzymes. The chickpea genotypes BCP 2010-1,
JG 2001-4, JG 24 and GL 23094 recorded higher phenol content and
higher level induction from pre to post infection in ß-1,3 glucanase,
chitinase and PAL activities. Chickpea genotypes BCP 2010-1 and JG
2001-4 recorded maximum fold increase in ß-1,3 glucanase activity with
5.30 and 5.28 fold respectively. Among the twenty four chickpea
genotypes screened under wilt sick soil three genotypes viz., BCP 2010-1,
JG24 and JG2001-4 recorded significant increase in activity profile of
four enzymes and total phenolics content with low field wilt incidence. The selected chickpea genotypes from wilt and drought
studies were screened using reported RAPD, ISSR and SSR primers. The
reported RAPD primers A07C and OPJ 20, ISSR primers ISSR 885 and
ISSR 855 and SSR primers TR 19 and TA 96 with specific amplicon size
have been validated for screening wilt resistance in chickpea while SSR
primers SSR 4 and CHK 03 which exhibited a specific amplicon size
fragment only in drought tolerant chickpea genotypes suggesting their
utility in chickpea drought screening.