Studies on shared biochemical and molecular responses to biotic (fusarium wilt) and abiotic (drought) stresses in Chickpea(Cicer arientium L)

Loading...
Thumbnail Image
Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Mahatma Phule Krishi Vidyapeeth, Rahuri
Abstract
The present investigation entitled “Studies on shared biochemical and molecular responses to biotic (Fusarium wilt) and abiotic (drought) stresses in chickpea (Cicer arietinum L.)” was undertaken to evaluate the activity profile of defense related enzymes and antioxidative enzymes during chickpea-Fusarium interaction and to correlate these changes with field infestation of chickpea in wilt sick soil. Total thirty one chickpea genotypes including four wilt resistant and three wilt susceptible checks were evaluated for total phenols and activity profile of defense related enzymes. The chickpea genotypes BCP 2010-1, JG 2001-4, JG 24 and GL 23094 recorded higher phenol content and higher level induction from pre to post infection in ß-1,3 glucanase, chitinase and PAL activities. Chickpea genotypes BCP 2010-1 and JG 2001-4 recorded maximum fold increase in ß-1,3 glucanase activity with 5.30 and 5.28 fold respectively. Among the twenty four chickpea genotypes screened under wilt sick soil three genotypes viz., BCP 2010-1, JG24 and JG2001-4 recorded significant increase in activity profile of four enzymes and total phenolics content with low field wilt incidence. The selected chickpea genotypes from wilt and drought studies were screened using reported RAPD, ISSR and SSR primers. The reported RAPD primers A07C and OPJ 20, ISSR primers ISSR 885 and ISSR 855 and SSR primers TR 19 and TA 96 with specific amplicon size have been validated for screening wilt resistance in chickpea while SSR primers SSR 4 and CHK 03 which exhibited a specific amplicon size fragment only in drought tolerant chickpea genotypes suggesting their utility in chickpea drought screening.
Description
Keywords
Citation
Collections