Rabies in animals: Post vaccinal serological response; and comparison of diagnostic techniques
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Date
2011
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GADVASU, Ludhiana
Abstract
The present study was conducted to elucidate the serological response in 180 dogs after vaccination and 3 rabies suspected cases in large animals by ELISA, presented to Department of Teaching Veterinary Clinical Complex, GADVASU, Ludhiana. Out of 180 dogs , 163 were immunized with different makes of rabies vaccine, 17 dogs were unvaccinated. In vaccinated dogs, rabies virus specific antibodies (above 0.5IU/µl) were detected in 71.16% (116/163) cases. None of the unvaccinated dogs and large animals showed protective rabies specific antibody titer. The antibody level was directly proportional to the number of vaccine shots. Vaccination in dewormed animals revealed better protection and antibody titre (8.878 IU/µl) than non dewormed ones (4.01 IU/µl). There was no significant difference in the rabies antibody titre among both the sexes. During the study period, out of 50 post mortem cases, 31 were diagnosed positive for rabies. The overall incidence of rabies was 62 per cent. Efficacy of different immuno-histological techniques in naturally occurring rabies cases was compared for the diagnosis of rabies, and was found to be 100% by immunohistochemistry and 83.33% by histopathology, respectively. For immunohistochemical studies polyclonal antiserum was found to be better than monoclonal antibodies. The amount of rabies viral antigen/number of Negri bodies detected with immunohistochemistry was much more abundant than could be detected by H & E staining. It was concluded that immunohistochemistry was more sensitive and a valid test and it can replace fluorescent antibody test in screening impression smears, where fluorescent microscope and fresh samples are not available