Analysis of innate immunity related genes in divergent germplasms of chicken

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Date
2012
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GADVASU, Ludhiana
Abstract
Although chicken strains show differences in susceptibility to a number of diseases the underlying immunological basis is yet to be elucidated. Avian heterophils have been reported to express toll-like receptor and beta-defensin genes. In the present study heterophils were subjected to LPS stimulation and total RNA extraction. Differential gene expression was studied in broiler, layer and indigenous Aseel strain by Real Time RT-PCR SYBR Green chemistry. The expression of the 14 AvBDs, chTLR 1, 2, 3, 4, 5, 7, 15 and 21 was detectable in heterophils. The expression level of most of the AvBDs and chTLR4 significantly increased (P<0.05) 3 hours post in vitro lipopolysaccharide challenge. Higher expression level and stronger activation of chTLR4, most AvBDs, NFkB-1 and IRF-3, in heterophils was observed, with the stimulation of LPS in layer compared to broiler, and in Aseel compared to both layer and broiler. The expression level of most of the AvBDs and chTLR4 significantly increased (P<0.05) 3 hours post in vitro lipopolysaccharide challenge. This investigation will allow more refined interpretation of immuno-genetic basis of the variable disease resistance/susceptibility in divergent stock of chicken including indigenous breed.
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