Study on inheritance of fertility restoration in brinjal (Solanum melongena L.)
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Date
2020
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Punjab Agricultural University, Ludhiana
Abstract
Brinjal, an often cross-pollinated crop is exploited in heterosis breeding due to advantages for
yield, horticultural and quality traits. However, hand emasculation and pollination process
makes the hybrid seed production costly, which can be reduced by using male sterility
mechanism. Recently, Department of Vegetable Science, PAU Ludhiana has developed male
sterile and restorer lines in brinjal. But the inheritance of fertility restoration was yet to
determine for further use in different breeding programs. Therefore, the present investigation
„Study on inheritance of fertility restoration in brinjal (Solanum melongena L.)‟ was planned,
where; four CMS (D-CMS 291A, D-CMS 99A, D-CMS5A and D-CMS 72A) and restorer
lines (R 2-1, R 3-4, R 6-2 and R 2596-2) lines were used to develop F1, F2 and BC1P2
populations. Phenotyping of all segregation populations and analysis with chi-square test
revealed involvement of single dominant gene (Rf) for fertility restoration. All the male fertile
plants exhibited normal anthers filled with pollen grains, while the sterile plants had
shrivelled anthers devoid of pollen grains. Further, pollen fertility analysis through pollen
stainability, pollen germination and pollen index indicated that restorer R 2-1 had highest
pollen fertility than the others. The germination media M5 containg 0.5% agar + 300ppm
calcium nitrate + 5% sucrose + 50mg/l boric acid + 400 mg/l PEG 4000 exhibited highest
pollen germination. The restorer R 2-1 also exhibited better floral and horticultural traits
compared with other three. Out of 29 primer pairs used, only 9 primers displayed
polymorphism between the parental DNA on 3% agarose and were then subjected to BSA
(Bulk segregant analysis). But, none of them distinguished between the male sterile and male
fertile bulks. This indicated that none of the primers used is linked to the Rf gene. For further
testimony, either these bulks can be subjected to QTLseqr or as now chromosome anchored
genome of brinjal is available, so new set of primers can be designed and validated to find
any closely linked marker to the fertility restorer gene.
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Citation
Khushwinder Kaur (2020). Study on inheritance of fertility restoration in brinjal (Solanum melongena L.) (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.