Studies on Wound Healing Potential of Bergenia ciliata Rhizome

Loading...
Thumbnail Image
Date
2019-10-28
Journal Title
Journal ISSN
Volume Title
Publisher
Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, Jammu-180009
Abstract
The present study was undertaken to evaluate the wound healing potential of ethanolic extract of rhizome of Bergenia ciliata on excision wound model. Rats weighing 150-200g divided into four groups of 10 animals each were subjected to various treatment regimens for 14 days to determine the effect of extract incorporated ointments on wound healing. Blood and tissue samples were collected after 7th and 14th days following daily topical application of simple, Povidone iodine and extract (5 & 10% w/w) ointments. Wound contraction was relatively quicker and higher with 10% extract ointment. Significant (P<0.05) increase in tissue hydroxyproline levels after application of extract or Povidone iodine ointments was observed. Glucosamine levels were restored to normal after 14 days of treatment after application of extract or Povidone iodine ointments. Total protein of blood treated with 10% extract and Povidone iodine ointment increased significantly (P<0.05) after 14 days of treatment. Significantly (P<0.05) increased SOD, CAT, GPx, GSH, GST & TTH levels in the blood of animals treated with 10% extract ointment was observed. AST and ALT levels decreased in all the treatment groups as compared to control. Higher decline in LDH levels was observed in Povidone iodine ointment group as compared to extract group. Pro-inflammatory cytokines (TNF-α and IL-6) levels were significantly (P<0.05) reduced after treatment with both 10% extract as well as Povidone iodine ointment. Histopathological studies on extract treated wounds revealed increased collagenation and re-epithelialization. These findings point towards the potential of ethanolic rhizome extract of B.ciliata in management of wounds.
Description
Keywords
Citation
Collections