STUDY ON QUALITY OF FROZEN SEMEN AND EFFICIENCY OF AI TECHNICIANS AND THEIR IMPACT ON REPRODUCTIVE PERFORMANCE OF BUFFALOES UNDER AI NETWORK PROGRAMME
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Date
2018
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DEPARTMENT OF ANIMAL REPRODUCTION GYNAECOLOGY AND OBSTETRICS POSTGRADUATE INSTITUTE OF VETERINARY EDUCATION AND RESEARCH KAMDHENU UNIVERSITY, GANDHINAGAR
Abstract
The research entitled “Study on Quality of Frozen Semen and Efficiency of AI
technicians and their impact on Reproductive Performance of Buffaloes under AI Network
Programme” was conducted to know the efficiency of AI technicians and reproductive
performance of buffaloes under AI network programme as well as to evaluate the post thaw
motility of spermatozoa by CASA.
The information on attributes of 83 AI technicians were collected which revealed that
highest percentage (38.6%) of the AI technicians were from 31 to 40 years of age group. The
percentage of AI technicians obtained higher secondary, graduation and above level of
education were 38.6 and 36.1, respectively. Seventy seven per cent of AI technicians had
undergone basic as well as refresher type of trainings which has contributed towards success
of AI. All the AI technicians have detected estrus prior to AI and all of them have observed
clear mucus discharge in estrus animals. Seventy four per cent of the AITs have confirmed
detection of heat by palpation of ovarian follicle. Ninety four per cent of the AITs have
practiced correct site of semen deposition.
Fifty per cent of the AI technicians have performed insemination at the ideal time
which resulted in significantly higher conception rate (46.18 ± 0.97) in buffaloes of
Sabarkantha district followed by Arvalli (41.02 ± 0.99) and Panchmahal (40.66 ± 0.99). Fifty
three per cent of the AI technicians have followed standard thawing temperature and time
(37°C and 30 seconds).
Sixty two frozen mini straws randomly selected from 31 AI technicians were
analysed for post-thaw motility, different velocities and sperm abnormalities. The overall
mean values of velocity (μm/s) of post-thawed spermatozoa of samples were: Average Path
Velocity (VAP) 100.86 ± 1.97, straightline velocity (VSL) 86.70 ± 1.76 and curvilinear
velocity (VCL) 157.10 ± 3.13. Further, the mean values of other velocity parameters of postthawed
sperms of samples were: ALH 5.98 ± 0.13 μm, BCF 35.53 ± 0.34 Hz, Straightness
82.66 ± 0.44 per cent, Linearity 55.48 ± 1.42 per cent, Elongation 56.23 ± 0.05 per cent and
Area 25.86 ± 0.43 μm2.
Straightness (STR) of the spermatozoa was found significantly higher (p<0.01) in
education group I, followed by groups II, IV and III. Similarly linearity (LIN) was found
significantly higher in group I followed by groups II, IV and III. The linear motility (59.88 ±
2.02) and wobbling motility (68.49 ± 1.45) were significantly higher in group IV than those
of groups I, II and III.
Highly significant difference was observed in the coiled tail characters of frozenthawed
spermatozoa in experienced groups of AI technicians. The difference in group I and
II were significantly lower than groups IV, III and V. Similarly the coiled tail count was
significantly higher in group IV followed by groups I, II, III and V.
Motile mean elongation, slow concentration, slow count, slow sample, total
concentration, total count and total sample were found significantly higher from the samples
collected from AITs who received basic training. The corresponding overall mean post-thaw
motility and progressive motility percentage of the above profile parameters were 73.59 ±
13.57 and 50.67 ± 1.52; 73.58 ± 13.57 and 50.67 ± 11.99; 73.59 ± 13.57 and 50.67 ± 11.99;
73.58 ± 13.57 and 50.67 ± 11.94; 73.59 ± 13.57 and 50.67 ± 11.99, respectively.
Age at first AI (months) in buffaloes of Sabarkantha district was lower than the age at
first AI for Arvalli and Panchmahal districts. Similarly the age at first calving (AFC) in
buffaloes of Sabarkantha district was lower than the buffaloes of Arvalli and Panchmahal
districts.
Present work on selected AI technicians’ profile and their employed practices had an
influence on AI success rates. Some of these positive influences of attributes have showed
influence on post-thaw motility and velocity parameters assessed by CASA. There is a dire
need to take up further studies in such buffaloes to correlate CASA traits of fresh and frozen
thawed semen with sperm motility, live sperm percentage and reproductive performance of
the buffaloes.