Analysis of genetic diversity of xanthomonas axonopodis pv. Citri using pcrbased molecular markers
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Date
2010-05-05
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Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
Abstract
Random amplified polymorphic DNA (RAPD) marker were used for
gemetic diversity analysis of Xanthomonas axonopodis pv. citri isolates. A
total of 13 RAPD primers were used. Amplification of Xanthomonas
axonopodis pv. citri Genomic DNA of strains, using RAPD analysis,
detected 153 amplicons that could be scored, of which 150 amplicons were
polymorphic, with an average polymorphic 11.53 amplicons per primers,
while monomorphic detected 3 bands, with an average 0.23 amplicons per
primer. The range of polymorphism per primer ranged from 7 bands 87.50
%or (OPA-13) to 18 bands or 100% (OPX-11). The size of amplification
products ranged from 50 bp (OPG-04) to 3000 (OP A-20, OPG-09 and
OPX-11). The average similarity coefficient value for 14 isoaltes were
found to be 0.302. the Xac-10 and Xac-11 shared highest similarity (55%).
The isoalte Xac-3 was most diverse with 88% diversity.