Induction of mutation for salinity tolerance in sugarcane cultivar (saccharum officinarum L.)
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Date
2010-08-18
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Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
Abstract
Salinity IS the major environmental stress, which greatly affect the plant
productivity. Breeding for salinity is difficult and hence the intervention of mutagenesis
and tissue culture can greatly facilitate the selection and isolation of useful tolerant lines.
In the present study, in vitro mutagenesis was employed in the selection of salt tolerant
lines in popular sugarcane (Saccharum officinarum L.) cv. CoC-94012. Embryogenic
callus cultures were subjected to UV radiation. The embryogenic callus cultures were
exposed to inhibitory levels ofNaCI (00, 25, 50, 75, 100 and 125 mM).
For callus induction and embryogenic callus production meristem and leaf base
segments were subjected to in vitro culture on MS medium supplemented with 4 mg I -I
2, 4, Dichlorophenoxyacetic acid for 4 weeks.
The result showed that a significant decrease in callus growth and cell viability
occurred with increasing levels of NaCI. Higher amounts of free proline and protein
were accumulated in NaCI stressed calli. Embyogenic sugarcane calli stressed with
different levels of N aCI showed the significant decreasing in levels of total soluble
sugar, with increasing the levels ofNaCl.