Identification and In Vitro Evaluation of Species Specific Probiotic for Feeding Broiler Chicken Using Probiotic Scores
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Date
2012
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Abstract
The study was undertaken to isolate and identify suitable species specific
probiotic for feeding broiler chicken. Probiotic organisms were obtained from
the gastrointestinal tracts of eight broiler chicken. Out of 24 bacterial isolates
from gastro intestinal tract, four were identified as Lactobacillus based on
morphological, physiological and biochemical tests which are specific for
Lactobacillus genus. These four Lactobacillus isolates were further identified at
species level as L.acidophilus, L.crispatus, L.salivarius and L.fermentum based
on sugar fermentation tests. The identified Lactobacilli species were screened
for probiotic properties by in vitro tests like acid tolerance, bile tolerance and
antimicrobial activity against E.Coli in agar well diffusion assay. ’Probiotics
score’ was considered as yardstick to identify the best species specific among
the probiotic organisms for feeding broiler chicken. ‘Probiotic score’ for
chicken, among the isolated Lactobacilli species, was formed by considering
the better Lactobacillus for acid tolerance at pH 2, bile tolerance at 0.3 per cent
bile acid in the MRS medium and antimicrobial activity against E.coli in agar
well diffusion assay. The best Lactobacillus species for acid tolerance, bile
tolerance and antimicrobial activity were L.acidophilus (optical density
2.000±0.001), L.fermentum (optical density of 0.218±0.010) and L.salivarius
(Inhibition zone 26 ±0.30 mm) respectively. Hence, from the results obtained
from Probiotic score it is concluded that L. salivarius which has the maximum
score (93.4/300) was chosen as best species specific probiotic and can be used
for feeding broiler chicks.
Description
TNV_IJVS_2012_1(2)64-68
Keywords
Veterinary Science