Role of Oxidative Stress Biomarkers on Embryonic Mortality in Bovines
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Date
2015
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Abstract
A total number of one hundred repeat breeder cows between second and fifth
calving were selected, and randomly and equally divided into five groups as
Group I to V. Cows of group I served as control and were inseminated during
natural oestrus. Cows of group II were treated for 3 weeks with intramuscular
injection of vitamin A, C and E. They were also supplemented with mineral
mixture continuously for 20 days. At the end of treatment, these cows were
observed for oestrus signs and inseminated at 16-18 hours after the onset of
oestrum. Cows of group III were inseminated at 16-18 hours after the onset of
natural oestrus and treated with an intramuscular injection of flunixin
meglumine at 12, 13, 14 and 15 days of post-insemination. Cows of group IV
were treated intravaginally with CIDR (Controlled internal drug release) for 9
days and following its removal, by observing oestrus signs, they were
inseminated. Cows of group V were administered with two injections of PGF2α
at 11 days interval and they were observed for oestrus signs after second
injection of PGF2α and inseminated during induced oestrus. The serum
oxidative biomarkers viz., reduced glutathione (GSH), superoxide dismutase
(SOD) and malondialdehyde (MDA) were estimated in pregnant cows of all the
groups on days 0, 5, 10, 15, 20, 30, 45 and 60 and non-pregnant cows on days 0,
5, 10, 15 and 20. Among all the treatment groups, the serum oxidative
biomarkers viz., GSH and SOD were higher in pregnant (day 0 to 60) and non
pregnant (day 0 to 20) cows treated with antioxidant cows (group II).
Comparing the pregnant and non pregnant cows in each group, GSH and SOD
were higher in pregnant cows than non pregnant cows from day 0 to 20. The
mean serum MDA was found to be lower in pregnant and non pregnant cows in
group II than in other groups.
Description
TNV_IJVS_2015_4(1)6-9
Keywords
Veterinary Science