Effects of Purified Saponin on Rumen Methanogenesis and Rumen Fermentation Characteristics Studied Using In Vitro Gas Production Technique
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Date
2013
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Abstract
An experiment was conducted to study the effects of purified saponin at the
level of 0, 1.55, 3.10, 4.65 and 6.20 mg/30 ml rumen inoculum in triplicate on
rumen methanogenesis and rumen fermentation characteristics by in vitro gas
production technique. The total gas production was significantly (P<0.01)
reduced in all saponin treated groups when compared to the control. The highest
level of reduction in total gas was observed with 4.65 mg saponin treatment
than other treatment groups. The methane production was significantly (P<0.01)
decreased in all saponin treated groups when compared to the control. The
highest level of methane reduction was observed with 6.2 mg saponin treatment
than other treatment groups. The decreases in methane reduction were 14.04 %,
21.90 %, 34.30 % and 37.60 % in 1.55, 3.10, 4.65 and 6.20 mg saponin levels
respectively than 0 mg saponin level. The percentage of methane on total gas
production was also significantly (P<0.01) reduced in all saponin treated groups
than the control. The highly significant (P<0.01) reduction was observed with
4.65 mg of saponin treatment and this treatment decreased the methane
percentage on total gas production by 15.04 % when compared to the other
treatment groups. The methane emission per 100 mg of digested substrate was
also significantly (P<0.01) reduced with 4.65 mg saponin treatment than other
treatment groups. No significant difference was observed on pH and ammonia
nitrogen levels in all the treatment groups. The in vitro true dry matter
digestibility (IVTDMD) was not affected by saponin and no significant
difference was observed. The protozoal count was significantly (P<0.05)
reduced in all saponin treated groups when compared to the control. The
reduction in protozoal counts were 14.40%, 15.56%, 19.31% and 23.05%
respectively with 1.55, 3.10, 4.65 and 6.20 mg saponin treatments when
compared to the control. The bacterial count in all saponin treated groups was
not significantly affected, however the bacterial counts showed a decreasing
trend with increased levels of saponin when compared to the control. No
significant difference was observed on total volatile fatty acids (TVFA), acetic
acid, propionic acid, butyric acid and acetate to propionate ratio in all saponin
treated groups when compared to the control. Based on our findings we
concluded that treatment with purified saponin reduces the total gas and
methane emission significantly without affecting the rumen fermentation
characteristics such as pH, ammonia nitrogen, IVTDMD, microbial load and
TVFA.
Description
TNV_IJVS_2013_2(2)44-49
Keywords
Veterinary Science