EFFECT OF IN OVO INJECTION OF PROBIOTIC, PREBIOTIC AND SYNBIOTIC ON THE PRODUCTION PERFORMANCE AND GUT HEALTH OF COMMERCIAL BROILER CHICKEN

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Date
2018
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Abstract
A biological experiment was carried out to evaluate the effect of in ovo injection of probiotic, prebiotic and synbiotic on broiler production performance, immune status and gut health of commercial broiler chicken upto 5 week of age. On d 18 of incubation, 400 eggs were randomly divided into 5 experimental groups of 80 eggs each and were injected with different bioactives viz., 0.2 ml of Lactobacillus acidophilus 3x107 cfu, 0.2 ml of 0.5% Mannan-oligosaccharide, 0.2 ml of Lactobaccilus acidophilus 3x107 cfu + 0.5% Mannan-oligosaccharide and were compared with non-injected control, sham (positive) control injected with 0.2 ml of Normal Saline. Each treatment had 4 replicates of 20 broilers each. All chicks were reared under deep litter system up to five weeks of age in a semi environmental control poultry house. Birds were fed ad libitum with pre starter, starter and finisher mash prepared as per BIS (2007) from 0-14 d, 15-21 d and 22-35 d of age, respectively. The data on hatchability, body weight and gain up to fifth week of age, cumulative feed consumption, feed to gain ratio, livability, pancreatic enzyme activity in serum, colonization of microbiota in gut, immune status related to New Castle Disease and Infectious Bursal Disease, relative gene expression of gut health and functionality related genes and relative economics involved in broiler chicken rearing including Broiler Performance Efficiency Factor (BPEF) and Broiler Feed Price Ratio (BFPR) were recorded, calculated and analysed. In ovo injected and non-injected control groups resulted in non-significant effect on hatchability. Intra amniotic supplementation had significant (P < 0.05) inference on body weight and body weight gain during fifth week of age. The fifth week cumulative feed consumption and FCR was not significantly (P < 0.05) changed by in ovo treatment. The weekly and cumulative livability of broiler was not significantly affected throughout the experimental period. The serum concentration of amylase, lipase and trypsin had significantly (P < 0.05) increased during third week of age in birds received in ovo L. acidophilus, MOS and their combination when compared with control. The colonization of microbiota was observed in in ovo L. acidophilus, MOS and their combination group when compared with control and sham control. Among treatments antibody titre values against ND virus and IBD virus on 7th and14th day of post vaccination was not influenced by in ovo injection of L. acidophilus and MOS separately or by their combination. The following MUC-2, SI, AP, PepT-1 and SGLT-1 gut heath and functionality related gene were up regulated in the ileum of broilers that received in ovo injection when compared with control. Broiler Farm Economy Index (BFEI) and Broiler Feed Price Ratio (BFPR) was high in broilers that received L. acidophilus, MOS and their combination via in ovo when compared to control and sham control.
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TNV_TH_MVM 16028_2018
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Veterinary Science
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