CYSTEINE AND GLUTATHIONE SUPPLEMENTATION ON THE QUALITY OF CRYOPRESERVED DOG SEMEN
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Date
2018
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Abstract
The present study was aimed at studying and comparing the effect of cysteine and glutathione supplementation on plasma membrane integrity, oxidative stress and lipid peroxidation in cryopreserved dog semen and determining the optimum concentration of the tested antioxidants (cysteine and glutathione) that counteracts the cryopreservation damage during freezing and thawing.
Semen was collected by digital manipulation from six male dogs and motility, viability, concentration, morphological abnormality, acrosomal integrity, plasma membrane integrity and DNA integrity were assessed. The semen samples were diluted in the ratio of 1:1 to 1:2 with TRIS-egg yolk extender depending on the semen concentration and divided into five equal aliquots, among which one was kept as control group without any antioxidant addition and other four were supplemented with different concentrations of antioxidants viz: cysteine (5mM and 10mM) and glutathione (1mM, 2mM). Then, cryopreservation was done by conventional method. After freeze thawing process, motility, viability, morphological abnormality, acrosomal
integrity, plasma membrane integrity and DNA integrity were assessed. Also lipid peroxidation and activity of antioxidant enzymes like catalase, superoxide dismutase and glutathione reductase were evaluated.
Description
TNV_TH_MVM 16041_2018
Keywords
Veterinary Science