MOLECULAR DETECTION OF BACTERIA OF MYCOBACTERIUM TUBERCULOSIS COMPLEX (MTBC) AND MYCOBACTERIUM AVIUM COMPLEX (MAC) IN INDIAN GREY MONGOOSE (Herpestes edwardsii)
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Date
2017
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD
Abstract
In India, TB is a highly prevalent disease, which causes high mortality among
humans. This study was undertaken to detect the presence of MTBC and MAC
organisms in free ranging Indian Grey Mongoose (Herpestes edwardsii) by polymerase
chain reaction (PCR). Twenty one faecal samples were collected from free-ranging
mongoose in Zoological Garden, Thiruvananthapuram. The DNA from the faecal
samples was extracted by Qiagen stool minikit. The extracted DNA was subjected to
PCR to detect any mycobacterium (PCR targeting 16S rRNA gene), MTBC (PCR
targeting IS6110 and MAC (PCR targeting IS1311). Out of 21 samples, 16 (76.19
percent) were found positive for mycobacteria. None of the samples were positive for
bacteria belonging to MTBC and MAC. Therefore it was concluded that the mongooses
in the selected area are free from any pathogenic mycobacteria of MTBC and MAC.
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