Identification and Validation of Gene Tagged SNP Markers linked to Yield Related Traits in Rice
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Date
2020
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Indira Gandhi Krishi Vishwavidyalaya, Raipur
Abstract
Rice is the most popular agricultural crop and is the main hub of many developing countries of Asia on the continent. It also used as a food source in the continent of Africa Rice production should be increased to meet the growing need of rice to feed the growing population increased genetic efficiency is an important measure for the efficiency of rice production. It is important to identify the genes to be improved to produce fresh mega rice through breeding programs based on rapid molecular marker for crop development.
In this study, F3 population lines were evaluated duringKharif season 2019 -2020 under irrigated condition and phenological data for 13 yield related traits were recorded. On the basis of mean performance, significant variation was observed in all the traits under irrigated condition. SNP markers flanking 2 yield related traits reported to explain high percentage phenotypic variation for their respective traits in 7 population from a cross MTU1010×WR151 and Swarna×RRE78, Swarna×WR1, Swarna×RRF78, MTU1010×WR162, MTU1010×WR-116, MTU1010×DJOGLON were used for validation in F3 population single marker analysis was carried out by single factor ANOVA. A total of their amplification band were obtained indicated the presence of two alleles for each parent. For DEP-1 (T/A) (Middle exon/ 5'- exon) the a band of size 600bp indicated expression in turn amplification of DEP-1 gene, where as a band of size 520bp indicated presence T/A SNP allele and 380bp showed SNP Allele. Similarly, for GHD-7 (G/A) (First exon) gene band of size 580bp indicated amplification of targeted gene, and size of 550bp and 400bp indicated expression and amplification of SNP alleles.
In the F3 population set, the marker DEP-1 showed a strong association of SWARNA×RRF78 with grain dimension traits such as flag leaf breath, biological yield, grain yield, explaining 0.6%, 23.12 %, 23.13 %, and MTU1010×WR-162 grain dimension 50% flowering, plant height, flage leaf length, grain length, grain breath, grain weight 100 seed, explaining 6.99%,6.49%,7%, 9.01% 5.9%, 4.51% of phenotypic variance, respectively. The marker-trait association for DEP-1 and were also found to be significant. Similarly In F3 population, the GHD-7 gene based marker MTU1010×WR-151 was found to be strongly associated with 50% flowering, no of tillers, grain length, grain weight 100 seed, explaining 0.17%, 14.05%, 0.34%, 2.2%, and SWARNA×RRF78 grain dimension 50% flowering, no of tillers, explaining 0.9%, 2.59%, and MTU1010×WR-116 grain dimension grain weight, explaining 0.3% of the phenotypic variance respectively at significant P values.
Description
Identification and Validation of Gene Tagged SNP Markers linked to Yield Related Traits in Rice
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