The high incidence of Repeat breeding is one of the most common problems faced by the farmers and veterinarians in India and it causes huge economic loss to the farmers. A total of 90 repeat breeder cows that were either brought to the Teaching Veterinary Clinical Complex, Veterinary College and Research Institute, Tirunelveli, or to the infertility camps at nearby villages of Tirunelveli formed the experimental animals for the present study. The repeat breeder cows were randomly and equally assigned to one of the three groups. viz. control (Group I; n=30) CIDR (Group II; n=30) and CIDR + post AI CIDR (Group III; n=30). The pregnancy status was checked 60 to 90 days post insemination by rectal palpation. The conception rate following controlled breeding with CIDR (group II) was (P<0.05) higher at 43.33 percent than untreated cows (Group I), with CIDR and post insemination treatment with CIDR (group III) was the highest at 63.33 percent than group I & II. Hence, Controlled breeding using CIDR and PGF2α in combination with post insemination CIDR therapy or alone could be used to improve the conception rate in repeat breeder cows under field conditions.