Studies in relation to molecular variability and stability in saffron (Crocus sativus L.)
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Date
2008
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SKUAST-K
Abstract
Stability analysis and molecular characterization using RAPD markers was carried out in 10 genotypes of saffron (Crocus sativus L) under three target environmental conditions of Kashmir valley. Observations were recorded on flowering, morphological, floral, yield and quality attributes. Significant variations were observed in genotypes for al the traits indicating the presence of high level of variability. Significant G x E interaction was observed for all the traits excepting days to 50 per cent sprouting and 50 per cent flowering. Thereby, revealing that genotypes perform differently for traits under study at different locations. Highly significant mean squares for environments excepting for flowering traits indicated that environment selected were random and were different in agroclimatic conditions. Partitioning of G x E interaction into linear and non-linear components indicated that G x E (linear) was observed to be significant for all the traits excepting for days to 50 per cent sprouting and days to 50 per cent flowering indicating that the behaviour of genotypes for these traits was predictable over the environments. Significant mean squares for pooled deviation (non-linear) in respect of days to 50 per cent flowering and corm yield plot-1 revealed that behaviour of deviation from regression existed among genotypes for these traits considering the desired population mean coupled with non-significant bi (equal to unity) and deviation form regression (S2di) equal to zero, two genotypes viz, SMD-45 and SD-3 were observed to be well adapted to all the environments. The results revealed that these genotypes observed average stability for all characters excepting for number of radical leaves and corm yield plot-1 (SD-3). Comparison of different stability of models in terms of their relative performance for different traits under study produced uniform information and ranked the genotypes without any major disagreement. SMD-45 was observed to be the best genotype having high quality coupled with high saffron yield.
Molecular characterization of ten saffron genotypes was carried out based on 21 Random Amplified Polymorphic DNA (RAPD) markers. The three conjugate paired primers generated 10 marker levels with 70 per cent polymorphism. The primer pair SA-D + SA-S found to be the best primer pair, which showed maximum number of scorable and polymorphic bands. The dendrogram based on molecular data showed considerable amount of genetic diversity among tested genotypes. The similarity index using Jaccard’s coefficient ranged from 0.375 to 0.834 and the highest similarity index (0.834) based on jaccard’s coefficient was observed between genotypes SMD-45 and SMD-79.
Description
M.Sc Thesis submitted to SKUAST Kashmir
Keywords
Saffron, Stability, RAPD analysis, Quality, Genetic and Plant Breeding