Biochemical characterization of saffron (Crocus sativus L.) germplasm
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Date
2007
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Publisher
SKUAST-K
Abstract
The present investigation was carried out to characterize the sixteen promising lines identified in saffron viz., SMD-3, SMD-6, SMD-30, SMD-45, SMD-61, SMD-68, SMD-81, SMD-115, SMD-116, SMD-117, SMD-138, SMD-140, SMD-211, SMD-224, SMD-235 and SMD-240. The lines were analysed for biochemical markers for four enzyme systems viz., catalase, lactate dehydrogenase, peroxidase and alcohol dehydrogenase. The analysis was carried out at two different stages i.e., active stage (leaf tissue) and dormant stage (dormant corm). Among the four enzyme studied for polymorphism, catalase showed a moderate level of distinctness among the lines followed by lactate dehydrogenase and alcohol dehydrogenase whereas, peroxidase showed least variability. During dormant stage, a total of eight, five, six and four bands were observed for Cat, Ldh, Per and Adh, respectively. On the other hand during the active stage a total of six, seven, six and five bands were observed for Cat, Ldh, Per and Adh respectively. Clustering pattern on the basis of biochemical markers supported the results obtained by the cluster analysis of the morphological traits. Although none of the enzyme systems differentiated all the lines, but a combination of two different enzymes could be used to characterize them. The present study can be taken as a bench mark for the subsequent investigation like DNA finger printing etc. that will provide precise information about the variability existing in the present lines.
Description
M.Sc Thesis submitted to SKUAST Kashmir
Keywords
Electrophoresis, biochemical markers, saffron, Genetic and Plant Breeding