Cultivar Characterization in Rice on the Basis of Morphological Make-up, Protein Profile and Isozyme Polymorphism
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Date
2005
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Publisher
SKUAST-K
Abstract
The investigation was carried out involving characterization of ten local rice genotypes including both the released (K-39, K-332, Jhelum, Chenab, Shalimar Rice-1, Kohsaar, Barkat) and pipeline (SKAU-337, SKAU-341, SKAU-382) cultivars. The genotypes were analysed for their morphological, chemical and bio-chemical markers in order to construct an identification key for seed production purposes and DUS testing. The genotypes could be categorized into distinct groups based on plant and seed morphological features. All the genotypes could be resolved on the basis of plant morphological traits whereas, only eight could be resolved on the basis of seed characters. Based on morphological markers at seedling stage, the genotypes could be categorized into two distinct groups with seven genotypes falling in one and three in the other group. On the basis of phenol colour reaction and KClO3 resistance test, eight genotypes could be distinguished. SDS-PAGE of seed proteins and alkaline PAGE of isozymes were found to be more reliable in characterizing the genotypes. Tris-soluble proteins extracted from dry seed showed a total of nineteen bands with relative mobility (Rm) values ranging from 0.32 to 1.00. The polymorphism for these bands was sufficient to identify the varieties as unique entities. Some of the bands were highly specific to a particular genotype particularly K-39 (Band 5), Chenab (Band 10) and SKAU-341 (Band 12). Among the four isozymes studied for polymorphism, esterase (EST), peroxidase (POX) and malate dehydrogenase (MDH) showed considerable polymorphism whereas, alcohol dehydrogenase (ADH) showed little polymorphism. A total of 14, 9, 10, and 6 bands were observed for EST, POX, MDH and ADH, respectively. Based on the descriptors used for characterization, the appropriate seed keys were developed that may prove helpful in establishing the genetic purity of the varieties in quality seed production programmes and an adjunct to testing for distinctness, uniformity, stability and novelty of rice varieties suitable for cultivation in Kashmir region.
Description
M.Sc Thesis submitted to SKUAST Kashmir
Keywords
Allozyme variation, Electrophoresis, KClO3 resistance test, Genetic and Plant Breeding