Seminal Attributes, Preservation and Fertilizing Ability of Refrigerated and Cryopreserved Semen of Berari Bucks

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Date
2020
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MAFSU, Nagpur
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The present investigation on “Seminal attributes, preservation and fertilizing ability of refrigerated and cryopreserved semen berari bucks” was conducted for study of scrotal biometry, evaluation of seminal characteristics, assessment of effect of dilutors for the preservation of buck semen at refrigeration temperature. The research was carried out on 144 semen samples of 6 bucks for various macroscopic, microscopic, sperm function tests and enzyme activity on neat semen and on semen diluted with in refrigerated semen Skim milk (SM) and Tris citric acid fructose egg yolk (TCFEY) dilutors while in cryopreserved semen TYG (Tris egg yolk glycerol) and TYD (Tris egg yolk di-methyl sulphoxide) diluters, at different stages of prefreezing and post freezing. The macroscopic, microscopic, sperm function tests and enzyme activity in neat semen of Berari bucks indicated that the quality of neat semen was very good and within normal prescribed limits. Similarly the microscopic, sperm function tests and enzyme activity in the semen of Berari bucks diluted with different diluters both in refrigerated with semen while in cryopreserved semen indicated that there was slight reduction in the quality of semen at every stage freezing of semen In refrigerated SM diluter was found to be superior than TCFEY While in cryopreserved semen TYG diluter was found to be superior than TYD at all the stages i.e. pre-freezing and post- freezing . In all diluters post thaw quality of semen was gradually reduced i.e. reduction in post thaw motility percentage and live sperm percentage, increase in sperm abnormality percentage, damage acrosome percentage and increase in enzyme leakage. This reduction in quality was within the prescribed limit. There were indications of increased leakage of enzymes into the plasma from pre-freezing to post thaw stage. Total 32 oestrous synchronized goats were inseminated with refrigerated and cryopreserved semen of Berari bucks. Conception rate of washed (removal of seminal plasma) semen of refrigerated or cryopreserved group was higher for Berari bucks. This indicates that washing plays an important role in semen preservation. Thus, on the basis of results obtained from the present research work, it can be concluded that the quality of diluted semen declines gradually during freezing of semen. However, this decline is gradual and within the normal permissible limits. The comparison between the results of washed and unwashed refrigerated or cryopreserved buck spermatozoa revealed that quality of semen was better in washed semen samples than in unwashed semen samples at each stages of freezing and gives better conception rate.
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