Protective Effect of Zingiber officinate and Its Combination with L-Ascorbic Acid on Induced Lead Toxicity in Poultry

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Date
2019
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MAFSU, Nagpur
Abstract
The present research work was carried out to investigate the extractability of plant Z. officinale in 50% ethanol, presence of active phytochemicals, safety evaluation in wistar rats and protective effect on induced lead toxicity in poultry. With 12% extractability, extract was dark brown in color and semi solid in consistency. The qualitative phytochemical screening revealed the presence of alkaloids, glyocosides, saponins, tannin and flavonoids, reducing sugars. GCMS analysis showed the presence of 12 major components with Butanoic acid, 2 methoxy methyl, Hexahydro farnesol, Gingerol and Tetradecanoic acid as main compounds. The safety evaluation (28days) of the extract was carried out. All the groups showed no alteration in all parameters studied except there was slight elevation in serum AST, ALT & ALP level with mild degenerative changes in liver & kidney at high dose (800mg/kg BW). The protective effect of extract was carried out on 140 Broiler chicks divided into seven groups of 20 birds each. On physical paramters Z. Officinale extract alone and with L-ascorbic acid has significantly suppressed the negative impact of lead on body weight and body weight gain, also by L-ascorbic acid alone. Similarly, significant improvement in feed conversion ratio (FCR) was observed however; there was not improvement in feed intake by all treatments. It was also observed that feeding of extract @ 200m/kg bw alone and L- ascorbic acid @ 200mg/kg feed alone has reduces the economic loss caused by lead toxicity and found to be more beneficial than its combination. The protective effects of Z. officinale aginst was noticed @ 200 and 300mg/kg bw alone and with L-ascorbic acid by restoring hematological and biochemical parameters. The lipid peroxidation caused by lead was significantly reduced by extract alone and with L-ascorbic acid as was proved by depletion in TBARS level with increased glutathione level in liver. The oxidative stress caused due to lead was suppressed by enhancing activities of antioxidant enzymes like glutathione peroxides, superoxide dismutase and catalase. Histopathological study revealed that 50% ethanolic extract alone and with ascorbic acid has ameliorated the pathological alterations by restoring the damage tissue of liver. From the investigation, it was concluded that 50% ethanolic extract of rhizomes of Z. officinale possesses remarkable phytochemical and bioactive compounds and with L-ascorbic acid has significant protective effect against the lead induced toxicity in poultry.
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