DOUBLE HAPLOID PRODUCTION AND IN-VITRO SCREENING OF DROUGHT TOLERANCE IN RICE
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Date
2020
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Indira Gandhi Krishi Vishwavidyalaya, Raipur
Abstract
In anther culture γ radiation is the mutation breeding tool used to obtained homozygous lines. The use of γ radiation increases the callus initiation and plant regeneration frequency. The present work was done to study the effect of γ radiation on callus initiation and plant regeneration of Dubraj. The callus was subjected to different doses of γ radiation viz 10,20,40,60,80 Gy along with control (0) respectively. When the radiation level increased, the maximum green callus percent was obtained at 10 Gy (25.6) as compared to control condition (12.7) and further decrease at 40,60 and 80 Gy.
Anther culture derived plants, were either haploid homozygous and diploid heterozygous to distinguish between them ploidy analysis was done to establish homozygosity. A flow cytometry method was used for ploidy determination which is an expensive and costly. To identify haploids and diploids in anther culture, cytological study was done in rice with the help of a light microscope which is a cheapest method. Thirty days old callus from haploid and diploid callus were taken for cell and nucleus size measurement. A result shows that nucleus size of haploid cell is nearly half of the diploid cell.
Drought decreases total biomass production by causing stress at vegetative and reproductive stagesin rice cultivars. Ricedouble haploid cross Swarna sub 1x IR-159 with a forty-one plant genotypes were screened in vitro at various concentrations of Polyethylene glycol (PEG) 6000-3.0, -5.0, -7.5, -10.0, -12.5 and control (0) bars of water potentialData were recorded on root length and shoot length of 12-15 days old seedling. Among all the double haploid lines, seven lines were found to be drought tolerant. At highest concentration -12.5, the healthy growth was observed with the mean root length (4.3) and shoot length (3.4).The genomic analysis reveals that obtained double haploid lines were genotypically drought tolerant by using SSR markers.
Description
DOUBLE HAPLOID PRODUCTION AND IN-VITRO SCREENING OF DROUGHT TOLERANCE IN RICE
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