MOLECULAR CHARACTERIZATION AND TYPING OF STAPHYLOCOCCUS ISOLATED FROM NOSTRILS OF FOOD HANDLERS
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Date
2016
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Abstract
The research work was carried out to investigate the nasal carriage rate of Staphylococcus spp. among healthy food handlers and to characterize the isolates using molecular methods. A total of 32 nasal swabs were collected food handlers. The collected samples were processed for isolation of Staphylococcus and the isolates were characterized using various PCR based assays targeting 16S rRNA, nucA, femB, mecA and classical enterotoxin genes. The isolates were then subjected to molecular typing by detection of polymorphism in coaA gene and by RAPD analysis using T3 and M13 primers. Results indicated that all the food handlers screened were colonized with Staphylococcus spp. and the S. aureus nasal carriage was found to be 25% of which one isolate was found to be methicillin resistant Staphylococcus aureus. Coagulase negative isolate were also found to carry the methicillin resistance gene and genes responsible for classical enterotoxin production. RAPD analysis revealed that multiple strains colonized the nostrils of the food handlers. This research work highlights the imminent risk of contamination of food materials by handlers therefore adoption of proper hygienic practices is important.
Description
TNV_JCTR_2016_16(3)5799-5810
Keywords
Veterinary Science