Changes in redox metabolism of barley (Hordeum vulgare L.) under drought stress
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Date
2020
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Publisher
Punjab Agricultural University, Ludhiana
Abstract
In the present investigation, effect of water stress on redox metabolism was evaluated in the
flag leaf and grains of four barley (Hordeum vulgare L.) genotypes viz DWRB 101, 432
ICARDA, Jyoti and 430 ICARDA at 10th, 20th and 30th DAA. Free radical scavenging
activities, relative water content, electrolyte leakage, antioxidative enzymes and their related
metabolites were studied during drought stress. The upregulation of CAT and POX at 20th
DAA while APX, GR, DHAR and PPO at 30th DAA in the flag leaf and grains of DWRB
101and 432 ICARDA may be responsible for lesser increase in H2O2 content as compared to
other genotypes. Moreover, the downregulation of SOD was comparatively higher in Jyoti
and 430 ICARDA. However, no significant change in NADPH oxidase activity was recorded
in all genotypes throughout the stages of plant development.The redox homeostasis was well
established during the stress in DWRB 101 and 432 ICARDA by maintaining comparatively
higher ratios of AsA/DHA and GSH/GSSG. Relatively stable water status and higher glycine
betaine, proline, choline and total phenolic content alongwith better radical scavenging
activities were observed in both the tissues of DWRB 101 and 432 ICARDA. Therefore,
scrutiny of data indicated that DWRB 101 and 432 ICARDA may perform better under
drought stress in comparison to Jyoti and 430 ICARDA.