Development of rapid flow-through-based dot-immunoassay for serodiagnosis of leptospirosis in dogs
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Date
2011
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Abstract
An IgG-ELISA used recombinant antigen and a rapid flow-through enzyme immunoassay
were developed for rapid screening of leptospiral antibodies in dogs using recombinant
LipL41, which is one of the conserved outer membrane proteins in pathogenic leptospires
as the coating antigen. Results from this study were compared with the standard
microscopic agglutination test and found that the sensitivity and specificity of the
enzyme-linked immunosorbent assay were 75.46% and 93.29% and whereas that of flow-
through-based dot-immunobinding assay were 87.73% and 89.63%, respectively. Relative
merits of these tests were also assessed. The flow-through-based dot-immunobinding
assay was thus proved to be a valid screening test for canine leptospirosis.
Description
TNV_CIMID_2011_34(1)17