A total population of community dogs 17.13 million has been reported all over the Indian states which act as a major source of infections for zoonotic diseases like rabies in humans and owned dogs. Failure of existing surgical sterilization method leads to development of anti-fertility vaccines as a new strategic method to control community dog population. In this present study efficiency of recombinant zona pellucida have been analyzed as immunogen for contraceptive vaccine, in this trial Group-A, B, C & D has four no’s of female dogs in each group were immunized (Group-A, B, C & D) were immunized with Alum control, TTKK- dZP3, TT-KK-dZP3-GnRH2 and physical mixture of TT-KK-pZP3 and bRNase–KK-pZP4, respectively. The anti-fertility of immunized dogs were assessed by monitoring of immunized female dogs for reproductive physiology, vaginal exfoliative cytology, plasma hormone profile (Estrodial and progesterone) and serum antibody titre. The contraceptive effect of immunized dogs in Group-A, B, C and D were 50%, 25%, 0 % and 25 %, respectively on 180 DPI. The Group- C and D showed more contraceptive efficacy in comparison with other two groups Group- A and Group-B. Hence, TT-KK-dZP3-GnRH2 and recombinant porcine zona pellucida proteins can be used as a candidate proteins for using as dog contraceptive vaccine.