Screening and Identification of E. coli pathotypes by Multiplex PCR
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Date
2020-02
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Abstract
The study was undertaken to screen the E. coli isolates available at Department of Veterinary Public
Health and Epidemiology by multiplex PCR to simultaneously group Enterohaemorrhagic E. coli (EHEC),
Enteropathogenic E. coli (EPEC) and Enterotoxigenic E. coli (ETEC) pathotypes. A total number of
36 presumptive colonies isolated from milk and various dairy products were confirmed by cultural and
biochemical tests according to Bergey’s manual of determinative bacteriology (Holt et al., 1994). The samples
found to be positive by culture and biochemical tests were further subjected to DNA extraction by using bio
basic molecular biology kit and genus specific PCR analysis was carried out targeting UspA gene (Chen and
Griffiths, 1998). The isolates were further analysed for the virulence genes by using multiplex PCR using the
primers specific for stx1, stx2, eae, bfp, stII and lt. (Vidal et al.2004). Among the 36 E.coli isolates, three were
positive for EPEC (482bp), one was positive for ETEC (129bp) and EHEC (584bp) two were positive for
both EPEC and EHEC.
Description
TNV_20thMVC_PP_Feb-2020_PA30
Keywords
Veterinary Science