Positive correlation between Somatic Cell Count and Beta – Casomorphin 7 level in cow milk
Loading...
Date
2020-02
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Somatic cell count (SCC) is an important trait indicating udder health and quality of milk. Proteolytic
enzymes like pepsin, elastase are released from somatic cells, lead to cleavage of β-casein into a seven amino
acid peptide, beta-casomorphin-7 (BCM-7). BCM-7 is structurally similar to morphine and exerts morphine
like effect in the consumers. BCM-7 levels and somatic cell counts were analyzed in milk samples (n= 100)collected from cattle of different β-casein genotypes (A1/A1, A1/A2, A2/A2). The results showed positive
correlation (R= 0.51) between SCC and BCM-7 level and 26% (R2 = 0.26) variance in the BCM-7 level was
explained by SCC. To confirm the role of SCC in BCM-7 level, somatic cells were isolated from milk and
different numbers (10000, 25000, 50000, 100k and 200k) of somatic cells were incubated with A1/A2 β–
casein (100ng/μl) and the levels of BCM–7 was estimated using a commercial ELISA kit. Positive correlation
(R= 0.87) was obtained between the SCC and BCM-7 level indicating the cleavage of β-casein in to BCM-7
as was hypothesized. SCC in cow milk explains 77% (R2 = 0.77) variance in the BCM-7 concentration. These
preliminary results indicated the impact of SCC on BCM-7 level and cow milk quality.
Description
TNV_20thMVC_PP_Feb-2020_OP16
Keywords
Veterinary Science