Skip navigation
Beta Version

Presentation

Complete genome sequence analysis of canine Parvovirus new 2a variant strain isolated from a dog with severe haemarrogic gastroenteritis in South India

en
18
0

Attachments [ 1 ]

TNV_20thMVC_PP_Feb-2020_OP03.pdf(63.62 kB)

(TNV_20thMVC_PP_Feb-2020_OP03)

More Details

National Symposium on Veterinary Research Priorities in Translational Animal Health, Production and Food Safety
2020-02
1
20th Indian Veterinary Congress, Annual Conference of IAAVR, TANUVAS

The canine parvovirus (CPV) infection is characterized by severe hemorrhagic gastroenteritis and myocarditis in dogs caused by different antigenic variants CPV-2a, CPV-2b, New CPV-2a, New CPV-2b and CPV-2c and these antigenic variants are evolved rapidly and completely replaced the original CPV-2 variants in throughout the world. In India, there are several reports even in vaccinated dogs with CPV infections in recent years and the predominant type of antigenic variant is CPV-2a. Here, we report a virulent field CPV-2a strain, ABT/CPV/MVC02, was isolated from a faecal sample of a dog with severe haemorrhagic gastroenteritis in Chennai, Tamil Nadu, India. The genome sequence of ABT/CPV/MVC02 was determined and analyzed to study the CPV-2 antigenic variants currently circulating in India. The genomic sequence of ABT/CPV/MVC02 comprises of 4495 nucleotides (nt) containing two open reading frames (ORFs). ORF1 (nt 1-2007) encoded two non structural proteins (NS1 and NS2), and ORF2 (nt 2014-4270) encoded two structural proteins (VP1 and VP2). The genome sequence of ABT/CPV/MVC02 shares 99.33% nucleotide identity with those of CPV new 2a variants (MH476591 and MH476580) strains and shares 99.20% and 98.98% nucleotide identity with JQ268284 (CPV 2b) and KU508407 (CPV 2c) strains respectively. There is one insertion site at VP1 region (nt- 2113) and two deletion sites were found at 3’ untranscribed region (UTR) (nt-4478 and 4481). The present study reveals that the ABT/CPV/MVC02 CPV-2a new variant strain circulating in India is evolving rapidly with unique variations at nucleotide level. Nucleotide sequence accession number: The genome sequence of new CPV-2a strain ABT/CPV/MVC 02 has been deposited in Gen Bank under the accession number MN661243.

TNV_20thMVC_PP_Feb-2020_OP03

Comments

(Leave your comments here about this item.)

Item Analytics

 
 

Select desired time period