GENETIC DIVERSITY ANALYSIS IN OAT (AVENA SATIVA) USING AGRO-MORPHOLOGICAL AND MOLECULAR MARKERS
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Date
2018
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CSKHPKV, Palampur
Abstract
The present investigation was undertaken to assess the extent of genetic variability and diversity among different oat genotypes using agro morphological and molecular markers so as to identify promising genotypes for future breeding programme. The experimental material consisting of 57 different genotypes of oat were raised in Randomized Block Design with three replications during the Rabi 2016-2017 and data were recorded on agro morphological, quality traits and reaction to powdery mildew resistance. Genetic diversity among different genotypes was studied on the basis of morphological traits using Mahalanobis D2-statistic, PCA and SSR markers.
Mean values for different traits revealed that genotypes JPO-36, JPO-46, JPO-38, JPO-17, JPO-29, JPO-30, JPO-31, JPO-45, EC-528883, Algerian, IG-03-203, EC-528889, EC-528890, EC-528390, JPO-28, OL-822, KRR-AK-36, EC-605834, PLP-14 , KRR-AK-26 and KRR-AK-42 were significantly superior for forage and quality traits and were resistant to powdery mildew. PCV along with GCV were high for tillers per plant, flag leaf area, leaf stem ratio, dry matter yield per plant and crude protein yield per plant. High heritability along with high genetic advance was observed for tillers per plant, dry matter yield per plant, crude protein yield per plant and seed yield per plant. Dry matter yield per plant, crude protein content, crude protein yield per plant and biological yield per plant were observed as best selection indices for green fodder yield whereas dry matter yield, biological yield per plant and harvest index were observed as best selection indices for seed yield.
Based on D2-statistic, all the genotypes were grouped into eight clusters. In contrast, SSR analysis grouped the genotypes into two clusters and further two sub-clusters. Genotypes PLP-16, PLP-1, JPO-50, JPO-31 JPO-28, PLP-17, EC-528897, JPO-38, EC-605831and JPO-29 were found to be more diverse than others.
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