VASCULAR PERFUSION AND MORPHOMETRY ANALYSIS OF PREOVULATORY FOLLICLE AND CORPUS LUTEUM DURING INDUCED OESTROUS CYCLE IN GRADED MURRAH BUFFALOES
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Date
2019-12
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Abstract
A total of 12postpartum pleuriparous cyclical graded Murrah buffaloes were randomly selected. All the
animals were inserted with Progesterone Impregnated Intra Vaginal Device(PIVD)on day 10 and on day 18,
500 μg of cloprostenol was injected intramuscularly. PIVD was removed 24 hours after PGF2α injection(day
19) and AI was done at 48 and 72 hours (Double AI) after PIVDwithdrawal. The vascular perfusionand
morphometry of the preovulatory follicle (POF), mature CL and early pregnancy CL was assessed by Doppler
ultrasonography.Statistical analysis revealed a high significant (P < 0.01) difference in the mean (± SE) POF
size during induced estrus between the pregnant (13.30 ± 0.20 mm) and non-pregnant buffaloes (11.18 ± 0.58
mm).At the time of induced estrus the mean (± SE) RCL size in buffaloes which became pregnant was lesser
than the non-pregnant buffaloes.A high significant difference (P < 0.01) was observed in the mean (± SE)CL size between pregnant and non-pregnant buffaloes on day 10and 28. At the time of induced estrus, there
was a high significant (P < 0.01) differencein all the mean (± SE) spectral indices of POF (PI, RI and TAMV)
between pregnant and non-pregnant buffaloes. On day 10 and 28 post AI, the mean (± SE) PI, RI and TAMV
values of CL of pregnant buffaloes significantly differed from non-pregnant buffaloes. Out of 12 animals 7
animals were conceived(58.33 %). It was concluded that induction of estrus with PIVD + PGF2α protocol
found to be an effective tool to achieve conception rate as that of natural estrus without the need for estrus
detection in buffaloes.Doppler sonography provide an estimate of the physiological status of follicles and
corpora lutea and supporting clinical diagnoses and management decisions (Embryonic mortality/Fetal loss).
Description
TNV_35thVCRI_CA_Dec-2019_IFBP-15
Keywords
Veterinary Science