Molecular and biochemical studies for yellow rust resistance in wheat (Triticum aestivum L. em. Thell)
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Date
2019
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CCSHAU
Abstract
The present study was carried out for evaluation of F3 and F4 generation progenies derived from a cross WH 711 (susceptible) x PBW698 (resistant) for identification and selection of better performing progenies for different characters along with yellow rust resistance. The experiment was carried out during the Rabi season of 2016-17 and 2017-18 in research area of Wheat and Barley Section, Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar. Data were recorded for Plant height (cm), Flag leaf length (cm), Flag leaf area (cm2), Spike length (cm), Spike weight (g), Number of grains/ spike, Grain weight/ spike, 100 grain weight (g), Number of tillers/plant, Grain yield/plant (g), Biological yield/plant (g), Harvest index (%), Days to heading and Reaction to yellow rust (Cobbe„s modified scale). High to moderate range of PCV and GCV were observed for traits number of tillers/ plant, grain yield/ plant, grain weight/ spike, spike weight and spike length. High heritability along with high genetic advance were recorded for number of tillers/ plant, grain yield/ plant and spike weight it indicates that this traits were govern by additive genetic variances. The correlation coefficient studies reveled that grain yield/ plant is significantly correlated with all yield attributing traits. The path analysis suggested that number of tillers/ plant, biological yield/ plant and harvest index maximum positive direct contribution towards grain yield/ plant in both the generation. D2 analysis grouped progenies in to 4 and 7 cluster in F3 and F4 generation. Principal component analysis reveled that the first 4 group contribute more than 60% of the variation. Grain weight/ spike, grain yield/ plant and number of tillers/ plant contribute more to the total variation. Out of 175 SSR primers used, 160 SSRs showed amplification in parental genotypes of PBW698 and WH 711 out of which, 40 SSRs were polymorphic and the remaining 120 gave monomorphic bands. The markers XGWM413, XPSP3000, XGWM140, XWMC44.1, XBARC181, XBARC76, and XGWM120 were associated with yellow rust. The marker XPSP3000 on chromosome 1B showed a maximum linkage with LOD value 10.47 explaining 6.28 % of phenotypic variation with an additive effect of 9.831. The Phenylalanine ammonia lyase (PAL) and Tyrosine ammonia lyase (TAL) activity ranged from 1.43 to 10.03 and 1.94 to 9.57 micro mol/ min/g FW respectively. The yellow rust was significant negatively correlated with PAL and TAL, whereas, Phenylalanine ammonia lyase and Tyrosine ammonia lyase was positively significant with each other.
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