INVESTIGATIONS ON ANTHRACNOSE (Colletotrichum gloeosporioides (Penz.) Penz. and Sacc.) OF POMEGRANATE (Punica granatum L.)

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Date
2007-07-30
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UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
Abstract
The severity of pomegranate anthracnose was more in Bagalkot district followed by Koppal, Bijapur and Raichur districts. The identity of the fungus was confirmed as Colletotrichum gloeosporioides (Penz.) Penz. and Sacc and deposited at NCFT, New Dehli. The isolates of C. gloeosporioides showed maximum growth on Potato dextrose agar and Richard’s broth on 13 th day after incubation at 27±1oC.There existed variability among eight isolates, of C. gloeosporioides with respect to type of the growth, mycelial color, pigmentation, size of the spore and sporulation. The highest radial growth and sporulation of the fungus was recorded at 30 oC, 100 per cent relative humidity and also light condition having 12 hours darkness alternated with 12 hours light. Among the fungicides, bioagents, botanicals and chemicals inducing systemic resistance were tested against the C. gloeosporioides of which difenoconazole, carbendazim + mancozeb, T. viride, eucalyptus leaf extract, salicylic acid and benzoic acid were superior, in inhibiting the mycelial growth of the fungus under in vitro condition. The bioefficacy of fungicides, bioagents and botanicals which performed well in vitro condition were tested in vivo condition as well. Among them, difenoconazole at 0.1 per cent concentration was effective in reducing the per cent disease index of anthracnose disease to the extent of 72.03% over control. Pre-harvest spray with 0.1 per cent difenoconazole or carbendazim showed least per cent disease index (23.23 &36.67 %) on 12 th day after storage. Post harvest dip with 0.1 per cent difenoconazole showed least per cent disease index followed by eucalyptus leaf extract on 12 th day after storage. Among the chemicals inducing systemic resistance, salicylic acid and benzoic acid were found more effective in reducing the per cent disease index with high total soluble solid, low total titrable acidity and less physiological loss in weight eight days after storage.
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