“Pathogenic Strains of Escherichia coli in Buffaloes and their Antibiotic Resistance with Special Reference to Extended Spectrum Beta Lactamases”
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Date
2018-10-15
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (J&K)
Abstract
The study aimed at finding theoccurrence ofpathogenic strains of Escherichia coli in buffaloes and their antibiotic resistance with special reference to extended spectrumbeta lactamases.Out of a total of 200E.coli isolates from faecal samplesfrombuffaloes, 43 isolates showed the presence of at least one virulence gene studied .Out of these 43 isolates 15(7.50%) isolates carried eae gene either alone or with ehxA gene and thus were detected as EPEC, while 25 (12.50%) isolates carried the stx gene and were detected as STEC. Among STEC isolates seven isolates carried stx2alone, nine carried stx2along with ehxA gene and four carried both stx1and stx2genesalong with ehxA gene,while five isolates carried eae and stx1gene. Three isolates carried ehxA gene alone.All the 43 E.coli isolates which carried any of the virulence genes were subjected to the screening test for detection of resistance to one or both of the twocephalosporinantibiotics (ceftazidime and cefotaxime).Out of 43 isolates five (11.64%) isolates were found to be resistant. Resistance to cefotaxime and ceftazidime was observed in four isolates (80.0%) each and three (60.0%) isolates showed resistance to both. Out of the five isolates that were positive in the screening test, only four (80.0 %) could be confirmed as ESBL producing by phenotypic confirmatory tests.All the isolates which were declared as ESBLs producers phenotypically were tested genotypicallyfor the presence of blaTEM, blaSHV and blaCTX-M genes by PCR.Three isolates were seen positive for blaCTX-Mand one isolate was found positive for blaTEM.
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